Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.87 extracted from

  • Jin, S.Y.; Skipwith, C.G.; Zheng, X.L.
    Amino acid residues Arg(659), Arg(660), and Tyr(661) in the spacer domain of ADAMTS13 are critical for cleavage of von Willebrand factor (2010), Blood, 115, 2300-2310.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E663A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme with substrate VWF73 peptide Homo sapiens
E664A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme with substrate VWF73 peptide Homo sapiens
G662A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme with substrate VWF73 peptide Homo sapiens
additional information deletion of amino acid residues Arg659-Glu664 from the ADAMTS13 spacer domain by site-directed mutagenesis results in dramatically reduced proteolytic activity toward von Willebrand factor73 peptides, guanidine-HCl denatured von Willebrand factor, and native von Willebrand factor under fluid shear stress, as well as ultralarge von Willebrand factor on endothelial cells Homo sapiens
R659A site-directed mutagenesis, the mutant shows altered activity compared to the wild-type enzyme with substrate VWF73 peptide Homo sapiens
R660A site-directed mutagenesis, the mutant shows altered activity compared to the wild-type enzyme with substrate VWF73 peptide Homo sapiens
Y661A site-directed mutagenesis, the mutant shows altered activity compared to the wild-type enzyme with substrate VWF73 peptide Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
VWF73 peptide pH 6.0, 37°C, recombinant Y661A mutant ADAMTS13 Homo sapiens
0.0018
-
VWF73 peptide pH 6.0, 37°C, recombinant wild-type ADAMTS13 Homo sapiens
0.0034
-
VWF73 peptide pH 6.0, 37°C, recombinant E664A mutant ADAMTS13 Homo sapiens
0.0035
-
VWF73 peptide pH 6.0, 37°C, recombinant E663A mutant ADAMTS13 Homo sapiens
0.0049
-
VWF73 peptide pH 6.0, 37°C, recombinant G662A mutant ADAMTS13 Homo sapiens
0.005
-
VWF73 peptide pH 6.0, 37°C, recombinant R660A mutant ADAMTS13 Homo sapiens
0.01
-
VWF73 peptide pH 6.0, 37°C, recombinant R659A mutant ADAMTS13 Homo sapiens
0.022
-
VWF73 peptide pH 6.0, 37°C, recombinant ADAMTS13 6-amino-acid-deletion mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens removal of newly released ultralarge-von Willebrand factor strings or bundles anchored on endothelial cells by ADAMTS13 occurs rapidly and efficiently in the presence and in the absence of fluid shear stress, suggesting that the cell-bound ultra large-von Willebrand factor polymers may be preferred substrates for ADAMTS13 ?
-
?
von Willebrand factor + H2O Homo sapiens ADAMTS13 cleaves von Willebrand factor at the Tyr1605-Met1606 bond von Willebrand factor fragments
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information removal of newly released ultralarge-von Willebrand factor strings or bundles anchored on endothelial cells by ADAMTS13 occurs rapidly and efficiently in the presence and in the absence of fluid shear stress, suggesting that the cell-bound ultra large-von Willebrand factor polymers may be preferred substrates for ADAMTS13 Homo sapiens ?
-
?
von Willebrand factor + H2O ADAMTS13 cleaves von Willebrand factor at the Tyr1605-Met1606 bond Homo sapiens von Willebrand factor fragments
-
?
von Willebrand factor + H2O ADAMTS13 cleaves von Willebrand factor at the Tyr1605-Met1606 bond. The ADAMTS13 spacer domain is required for cleavage of von Willebrand factor, role of the amino acid residues Arg659, Arg660, and Tyr661 of theADAMTS13 spacer domain in substrate recognition, sequence comparisons and kinetics, detailed overview Homo sapiens von Willebrand factor fragments
-
?
VWF73 peptide + H2O von Willebrand factor-derived peptide substrate Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More epitope mapping of anti-ADAMTS13 immunoglobulin G from patients with thrombotic thrombocytopenic purpura and sequence alignment of the ADAMTS13 spacer domains of human, mouse, and zebrafish Homo sapiens

Synonyms

Synonyms Comment Organism
ADAMTS13
-
Homo sapiens
More ADAMTS13 is a member of a disintegrin and metalloprotease with thrombospondin type 1 repeats, ADAMTS, family Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.76
-
VWF73 peptide pH 6.0, 37°C, recombinant Y661A mutant ADAMTS13 Homo sapiens
1.09
-
VWF73 peptide pH 6.0, 37°C, recombinant R660A mutant ADAMTS13 Homo sapiens
1.32
-
VWF73 peptide pH 6.0, 37°C, recombinant wild-type ADAMTS13 Homo sapiens
1.43
-
VWF73 peptide pH 6.0, 37°C, recombinant R659A mutant ADAMTS13 Homo sapiens
1.55
-
VWF73 peptide pH 6.0, 37°C, recombinant E663A mutant ADAMTS13 Homo sapiens
1.56
-
VWF73 peptide pH 6.0, 37°C, recombinant ADAMTS13 6-amino-acid-deletion mutant Homo sapiens
1.63
-
VWF73 peptide pH 6.0, 37°C, recombinant E664A mutant ADAMTS13 Homo sapiens
2.05
-
VWF73 peptide pH 6.0, 37°C, recombinant G662A mutant ADAMTS13 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
peptide substrates Homo sapiens
7.5 8 multimeric substrate Homo sapiens

General Information

General Information Comment Organism
physiological function the proteolytic cleavage of von Willebrand factor by ADAMTS13 appears to be necessary to eliminate ultralarge von Willebrand factor multimers in circulation, thereby preventing the formation of platelet-rich thrombi in small arterioles and capillaries Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
74
-
VWF73 peptide pH 6.0, 37°C, recombinant ADAMTS13 6-amino-acid-deletion mutant Homo sapiens
140.1
-
VWF73 peptide pH 6.0, 37°C, recombinant R659A mutant ADAMTS13 Homo sapiens
219.2
-
VWF73 peptide pH 6.0, 37°C, recombinant R660A mutant ADAMTS13 Homo sapiens
418.4
-
VWF73 peptide pH 6.0, 37°C, recombinant G662A mutant ADAMTS13 Homo sapiens
444.3
-
VWF73 peptide pH 6.0, 37°C, recombinant E663A mutant ADAMTS13 Homo sapiens
447.9
-
VWF73 peptide pH 6.0, 37°C, recombinant Y661A mutant ADAMTS13 Homo sapiens
487.7
-
VWF73 peptide pH 6.0, 37°C, recombinant E664A mutant ADAMTS13 Homo sapiens
733
-
VWF73 peptide pH 6.0, 37°C, recombinant wild-type ADAMTS13 Homo sapiens