Cloned (Comment) | Organism |
---|---|
expression of wild-type ADAMTS13 with a C-terminal Myc/His tag in HEK293 cells, transient expression of His-tagged truncated DAMTS13 mutants MP-Dis and MP and point mutants in HEK-293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D330A | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
D340A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
D343A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
L350G | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
L351G | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
additional information | construction of truncated DAMTS13 mutants MP-Dis and MP, which have a molecular weight of 30 kDa and 40 kDa, respectively | Homo sapiens |
Q333A | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Homo sapiens |
R349A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme, the mutant enzyme shows increased activity with the mutant D1614A von Willebrand factor115 substrate compared to the wild-type enzyme | Homo sapiens |
V352G | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of ADAMTS13 disintegrin-like domain mutants | Homo sapiens | |
0.09 | - |
VWF115 | pH 7.8, 37°C, recombinant mutant R349A | Homo sapiens | |
0.37 | - |
VWF115 D1614A mutant | pH 7.8, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.47 | - |
VWF115 D1614A mutant | pH 7.8, 37°C, recombinant mutant R349A | Homo sapiens | |
0.59 | - |
VWF115 | pH 7.8, 37°C, recombinant wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Zn2+ | required, metalloprotease | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
190000 | - |
x * 190000, recombinant wild-type enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
von Willebrand factor + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type ADAMTS13 with a C-terminal Myc/His tag from HEK293 cells, and recombinant His-tagged truncated DAMTS13 mutants MP-Dis and MP by nickel affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | role for ADAMTS13 disintegrin-like domains in substrate recognition and proteolysis, homology modeling, overview. Residues Arg349, Leu350, and Val352 are predicted to form a cluster on the surface of the ADAMTS13 disintegrin-like domain immediately adjacent to the active-site cleft | Homo sapiens | ? | - |
? | |
von Willebrand factor + H2O | - |
Homo sapiens | ? | - |
? | |
VWF115 + H2O | VWFA2 domain fragment, spanning von Willebrand factor residues 1554-1668, generation of 2 cleavage products of 10 kDa and 7 kDa | Homo sapiens | 10 kDa VWF115 fragment + 7 kDa VWF115 fragment | - |
? | |
VWF115 D1614A mutant + H2O | Asp1614 VWFA2 domain fragment, spanning von Willebrand factor residues 1554-1668, generation of 2 cleavage products of 10 kDa and 7 kDa | Homo sapiens | 10 kDa VWF115 fragment + 7 kDa VWF115 fragment | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 190000, recombinant wild-type enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ADAMTS13 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
VWF115 | pH 7.8, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.0062 | - |
VWF115 | pH 7.8, 37°C, recombinant mutant R349A | Homo sapiens | |
0.008 | - |
VWF115 D1614A mutant | pH 7.8, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.00875 | - |
VWF115 D1614A mutant | pH 7.8, 37°C, recombinant mutant R349A | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
VWF115 | pH 7.8, 37°C, recombinant mutant R349A | Homo sapiens | |
50 | - |
VWF115 D1614A mutant | pH 7.8, 37°C, recombinant wild-type enzyme | Homo sapiens | |
57 | - |
VWF115 D1614A mutant | pH 7.8, 37°C, recombinant mutant R349A | Homo sapiens | |
324 | - |
VWF115 | pH 7.8, 37°C, recombinant wild-type enzyme | Homo sapiens |