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Literature summary for 3.4.24.87 extracted from
- Partial purification and characterization of a protease from human plasma cleaving von Willebrand factor to fragments produced by in vivo proteolysis (1996), Blood, 87, 4223-4234.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | low salt concentrations activate the proteolytic activity | Homo sapiens | |
| Urea | activation, substrate is degraded at 1 M | Homo sapiens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | weak | Homo sapiens | |
| EDTA | complete inhibition | Homo sapiens | |
| EGTA | complete inhibition | Homo sapiens | |
| additional information | no inhibition by iodoacetamide, leupeptin, and serine protease inhibitorsDFP, PMSF, aprotinin | Homo sapiens | |
| N-ethylmaleimide | slow and weak inhibition | Homo sapiens | |
| Z-Phe-Phe-CHN2 | best peptidyl diazomethyl ketone inhibitor | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | plasma | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | best activating metal ion | Homo sapiens | |
| Ca2+ | activating | Homo sapiens | |
| Mg2+ | slightly activating | Homo sapiens | |
| additional information | no activtion by Zn2+, Cu2+, Cd2+, Ni2+, Co2+, Mn2+ | Homo sapiens | |
| Sr2+ | activating | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 300000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| proteins + H2O | Homo sapiens | - |
peptides | - |
? | |
| von Willebrand factor + H2O | Homo sapiens | cleavage of peptide bond Tyr842-Met843, large hemostatically active multimers are cleaved to smaller less active forms, increased proteolytic degradation in patients suffering from von Willebrand disease typ 2A | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| about 10000fold | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | not in platelet | Homo sapiens | - |
| plasma | - |
Homo sapiens | - |
| serum | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with human fibrinogen, bovine serum albumin, and calf skin collagen | Homo sapiens | ? | - |
? | |
| proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
| von Willebrand factor + H2O | cleavage of peptide bond Tyr842-Met843 | Homo sapiens | 2 peptides | - |
? | |
| von Willebrand factor + H2O | cleavage of peptide bond Tyr842-Met843, large hemostatically active multimers are cleaved to smaller less active forms, increased proteolytic degradation in patients suffering from von Willebrand disease typ 2A | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 x 300000, nonreducing SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M12.241 | Merops-ID | Homo sapiens |
| von-Willebrand factor degrading protease | - |
Homo sapiens |
| vWF-degrading protease | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
stable for several days during purification at room temperature | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
no activity at neutral pH at low salt concentration | Homo sapiens |
| 8 | - |
in presence of Ba2+ | Homo sapiens |
| 9 | 10 | in presence of Ca2+ | Homo sapiens |
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