Cloned (Comment) | Organism |
---|---|
expressed using the baculovirus expression system | Homo sapiens |
recombinantly expressed | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methoxycoumarin-4-yl-acetyl-Lys-Pro-Leu-Gly-Pro-Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg + H2O | - |
Homo sapiens | ? | - |
? | |
pN-collagen I substrate + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAM17 | - |
Homo sapiens |
ADAMTS-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Homo sapiens |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | transient transfection with siRNAs targeting ADAM17, which ablates its expression by up to 90%, inhibits phenyl mercuric acetate-stimulated shedding activity by up to 70%, indicating that ADAM17 is the major sheddase | Homo sapiens |
physiological function | a cell-based ADAM17 assay is used to show that thiol isomerases, specifically protein disulfide isomerase, could be responsible for maintaining ADAM17 in an inactive form. Down-regulation of thiol isomerases, by changes in the redox environment markedly enhances ADAM17 activation | Homo sapiens |