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Literature summary for 3.4.24.86 extracted from

  • Willems, S.H.; Tape, C.J.; Stanley, P.L.; Taylor, N.A.; Mills, I.G.; Neal, D.E.; McCafferty, J.; Murphy, G.
    Thiol isomerases negatively regulate the cellular shedding activity of ADAM17 (2010), Biochem. J., 428, 439-450.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed using the baculovirus expression system Homo sapiens
recombinantly expressed Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-yl-acetyl-Lys-Pro-Leu-Gly-Pro-Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg + H2O
-
Homo sapiens ?
-
?
pN-collagen I substrate + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ADAM17
-
Homo sapiens
ADAMTS-2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
assay at Homo sapiens
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens
7.5
-
assay at Homo sapiens

General Information

General Information Comment Organism
malfunction transient transfection with siRNAs targeting ADAM17, which ablates its expression by up to 90%, inhibits phenyl mercuric acetate-stimulated shedding activity by up to 70%, indicating that ADAM17 is the major sheddase Homo sapiens
physiological function a cell-based ADAM17 assay is used to show that thiol isomerases, specifically protein disulfide isomerase, could be responsible for maintaining ADAM17 in an inactive form. Down-regulation of thiol isomerases, by changes in the redox environment markedly enhances ADAM17 activation Homo sapiens