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Literature summary for 3.4.24.86 extracted from

  • Jin, G.; Huang, X.; Black, R.; Wolfson, M.; Rauch, C.; McGregor, H.; Ellestad, G.; Cowling, R.
    A Continuous fluorimetric assay for tumor necrosis factor-alpha converting enzyme (2002), Anal. Biochem., 302, 269-275.
    View publication on PubMed
Search for more literature for 3.4.24.86

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystal structure of the catalytic domain of TACE Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-[DL-[2-(hydroxyamino-carbonyl)methyl]-4-methylpentanoyl]-L-3-(tert-butyl)glycyl-L-alanine,2-aminoethylamide
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 NH2-LAQAVRSSSR-OH
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4',5'-dimethoxyfluoresceinyl-SPLAQAVRSSSR-cys(4-(3-succinimid-1-yl)fluorescein)-NH2 + H2O
-
 Homo sapiens ?
-
 ?
LAQAVRSSSR + H2O fluorimetric assay for TACE, fluorogenic substrate, a 10-amino-acid peptide capped with an o-aminobenzoyl group on the N-terminal end and with a 3-(2,4-dinitrophenyl)-L-2,3-diaminopropionic amide group on the C-terminal end, enzymatic conversion of the substrate results in a fluorescence enhancement of 11fold Homo sapiens ?
-
 ?
Mca-PLAQAV-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide-RSSSR-NH2 + H2O
-
 Homo sapiens ?
-
 ?
additional information o-aminobenzoyl-LAQVARSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl diaminopropionic amide is no substrate Homo sapiens ?
-
 ?
NH2-LAQAVRSSSR-OH + H2O uncapped counterpart of the fluorogenic substrate Homo sapiens ?
-
 ?
o-aminobenzoyl-LAQAFRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide + H2O enzymatic cleavage 22% Homo sapiens o-aminobenzoyl-LAQA + FRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide
-
 ?
o-aminobenzoyl-LAQAIRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide + H2O enzymatic cleavage 66% Homo sapiens o-aminobenzoyl-LAQA + IRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide
-
 ?
o-aminobenzoyl-LAQALRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide + H2O enzymatic cleavage 92% Homo sapiens o-aminobenzoyl-LAQA + LRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide
-
 ?
o-aminobenzoyl-LAQAVRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide + H2O enzymatic cleavage 90% Homo sapiens o-aminobenzoyl-LAQA + VRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide
-
 ?
o-aminobenzoyl-LAQFVRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide + H2O enzymatic cleavage 39% Homo sapiens o-aminobenzoyl-LAQF + RSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide
-
 ?
o-aminobenzoyl-LAQGVRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide + H2O enzymatic cleavage 11% Homo sapiens o-aminobenzoyl-LAQG + VRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide
-
 ?
o-aminobenzoyl-LAQLVRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide + H2O enzymatic cleavage 34% Homo sapiens o-aminobenzoyl-LAQL + VRSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide
-
 ?
o-aminobenzoyl-LAQVARSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide + H2O enzymatic cleavage 19% Homo sapiens o-aminobenzoyl-LAQVA + RSSSR-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl amide
-
 ?
proTNF-alpha + H2O
-
 Homo sapiens soluble TNF-alpha
-
 ?
transforming growth factor alpha + H2O
-
 Homo sapiens ?
-
 ?