Application | Comment | Organism |
---|---|---|
synthesis | in absence of cholesterol, SREBP double cleavage leads to activation of transcription of genes encoding multiple enzymes of the cholesterol biosynthetic pathway | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in M19 cell line | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D467N | mutation of aspartate abolish activity suggesting that is the third residue that coordinates the zinc active site of S2P | Homo sapiens |
N337Q | mutation at glycosylation site N337 does not abolish activity | Homo sapiens |
N508Q | mutation does not abolish activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
SREBP NH2-522 fragment + H2O | Homo sapiens | - |
NH2-484 fragment + 38 aa fragment | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43462 | s2p cloned | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | cDNA library | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
SREBP NH2-522 fragment + H2O | - |
Homo sapiens | NH2-484 fragment + 38 aa fragment | - |
? | |
SREBP NH2-522 fragment + H2O | cleavage at site-2 between 484-485 aa of SREBP cannot occur without prior cleavage at site-1 and is dependent on 478DRSR sequence | Homo sapiens | NH2-484 fragment + 38 aa fragment | - |
? |