Inhibitors | Comment | Organism | Structure |
---|---|---|---|
o-phenanthroline | inhibition of recombinant Ste24 CAAX proteolytic activity | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
integral to membrane | Ste24 has a lumenal N-terminus and a cytosolic C-terminus indicativ of an odd number of transmembrane spans, hydropathy analysis suggests 7 membrane spans | Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | restores Ste24 CAAX proteolytic activity after 1,10-phenanthroline treatment, reactivation with 0.25 mM Co2+ is 25% of that seen with 0.25 mM Zn2+ | Saccharomyces cerevisiae | |
Zn2+ | - |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Ste24, nickel chelate chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
YIIKGVFWDPA(farnesyl)CVIA + H2O | farnesylated 15-mer peptide containing the mature a-factor sequence and the native a-factor CAAX motif | Saccharomyces cerevisiae | YIIKGVFWDPA(farnesyl)C + Val-Ile-Ala | - |
? |