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Literature summary for 3.4.24.83 extracted from

  • Lo, S.Y.; Saebel, C.E.; Mapletoft, J.P.J.; Siemann, S.
    Influence of chemical denaturants on the activity, fold and zinc status of anthrax lethal factor (2015), Biochem. Biophys. Rep., 1, 68-77 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus megaterium Bacillus anthracis

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride
-
Bacillus anthracis
guanidine isothiocyanate
-
Bacillus anthracis
MgSO4
-
Bacillus anthracis
Na2SO4
-
Bacillus anthracis
NaCl
-
Bacillus anthracis
NaSCN
-
Bacillus anthracis
Urea
-
Bacillus anthracis

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mitogen-activated protein kinase kinase 1 + H2O Bacillus anthracis
-
?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus anthracis P15917
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-GYbetaARRRRRRRRVLR-4-nitroanilide + H2O
-
Bacillus anthracis ?
-
?
mitogen-activated protein kinase kinase 1 + H2O
-
Bacillus anthracis ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2 5 MgSO4 at pH 7.4 and 25°C Bacillus anthracis
50
-
Na2SO4 at pH 7.4 and 25°C Bacillus anthracis
140
-
guanidine hydrochloride at pH 7.4 and 25°C Bacillus anthracis
140
-
guanidine isothiocyanate at pH 7.4 and 25°C Bacillus anthracis
150
-
NaCl at pH 7.4 and 25°C Bacillus anthracis
150
-
NaSCN at pH 7.4 and 25°C Bacillus anthracis
1050
-
Urea at pH 7.4 and 25°C Bacillus anthracis