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Literature summary for 3.4.24.83 extracted from

  • Saebel, C.E.; Carbone, R.; Dabous, J.R.; Lo, S.Y.; Siemann, S.
    Preparation and characterization of cobalt-substituted anthrax lethal factor (2011), Biochem. Biophys. Res. Commun., 416, 106-110.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus megaterium Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+ is capable of directly replacing lethal factors active site Zn2+ to yield a hyperactive enzyme with 2fold higher kcat value and about 2.5fold increased catalytic efficiency Bacillus anthracis
Zn2+ dependent on Zn2+, contains 0.03 mol Zn2+ per mol of protein Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography and Superdex 200 gel filtration Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Gly-Tyr-betaAla-RRRRRRRRVLR-4-nitroanilide + H2O
-
Bacillus anthracis ?
-
?

Synonyms

Synonyms Comment Organism
anthrax lethal factor
-
Bacillus anthracis