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Literature summary for 3.4.24.83 extracted from
- Subsite specificity of anthrax lethal factor and its implications for inhibitor development (2011), Biochem. Biophys. Res. Commun., 407, 400-405.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (D-R)9LPY-CO-NHOH | - |
Bacillus anthracis |  |
| (D-R)9VLR-CO-NHOH | - |
Bacillus anthracis | |
| (D-R)9WLM-CO-NHOH | - |
Bacillus anthracis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mca-KKPTPIQLN-Dnp + H2O | Bacillus anthracis | - |
Mca-KKPTP + IQLN-Dnp | - |
? | |
| Mca-KKVYPYPMEK-Dnp + H2O | Bacillus anthracis | - |
Mca-KKVYP + YPMEK-Dnp | - |
? | |
| Mca-KKWLMYPLEK-Dnp + H2O | Bacillus anthracis | - |
Mca-KKWLM + YPLEK-Dnp | - |
? | |
| additional information | Bacillus anthracis | lethal factor cleaves it substrates between P1 and P1 and has a broad specificity with preference toward hydrophobic residues, but not charged or branched residues. The most preferred residues are, from P1 to P3, Trp, Leu, Met, Tyr, Pro, and Leu | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mca-KKPTPIQLN-Dnp + H2O | - |
Bacillus anthracis | Mca-KKPTP + IQLN-Dnp | - |
? | |
| Mca-KKVYPYPMEK-Dnp + H2O | - |
Bacillus anthracis | Mca-KKVYP + YPMEK-Dnp | - |
? | |
| Mca-KKWLMYPLEK-Dnp + H2O | - |
Bacillus anthracis | Mca-KKWLM + YPLEK-Dnp | - |
? | |
| additional information | lethal factor cleaves it substrates between P1 and P1 and has a broad specificity with preference toward hydrophobic residues, but not charged or branched residues. The most preferred residues are, from P1 to P3, Trp, Leu, Met, Tyr, Pro, and Leu | Bacillus anthracis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| anthrax lethal factor | - |
Bacillus anthracis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000028 | - |
(D-R)9WLM-CO-NHOH | in 0.1 M HEPES, pH 7.4 at 37°C | Bacillus anthracis | |
| 0.00000145 | - |
(D-R)9VLR-CO-NHOH | in 0.1 M HEPES, pH 7.4 at 37°C | Bacillus anthracis | |
| 0.00000732 | - |
(D-R)9LPY-CO-NHOH | in 0.1 M HEPES, pH 7.4 at 37°C | Bacillus anthracis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | anthrax lethal factor of Bacillus anthracis is a major factor for lethality of anthrax infection | Bacillus anthracis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000025 | - |
Mca-KKPTPIQLN-Dnp | in 0.1 M HEPES, pH 7.4 at 37°C | Bacillus anthracis | |
| 0.0013 | - |
Mca-KKVYPYPMEK-Dnp | in 0.1 M HEPES, pH 7.4 at 37°C | Bacillus anthracis | |
| 0.0023 | - |
Mca-KKWLMYPLEK-Dnp | in 0.1 M HEPES, pH 7.4 at 37°C | Bacillus anthracis | |
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