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Literature summary for 3.4.24.82 extracted from

  • Tortorella, M.D.; Burn, T.C.; Pratta, M.A.; Abbaszade, I.; Hollis, J.M.; Liu, R.; Rosenfeld, S.A.; Copeland, R.A.; Decicco, C.P.; Wynn, R.; Rockwell, A.; Yang, F.; Duke, J.L.; Solomon, K.; George, H.; Bruckner, R.; Nagase, H.; Itoh, Y.; Ellis, D.M.; Ross, H.; Wiswall, B.H.; Murphy, K.; Hillman, M.C., Jr.; Hollis, G.F.; Arner, E.C.; et al.
    Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins (1999), Science, 284, 1664-1666.
    View publication on PubMed
Search for more literature for 3.4.24.82

Cloned(Commentary)

Cloned (Comment) Organism
expression of cDNA in Drosophila S2 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
BB-16 0.000548 mM, 50% inhibition Bos taurus
BB-16 0.000159 mM, 50% inhibition Homo sapiens
SE206 0.000137 mM, 50% inhibition Bos taurus
SE206 0.000076 mM, 50% inhibition Homo sapiens
XS309 0.002185 mM, 50% inhibition Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aggrecan + H2O Bos taurus
-
 fragments of aggrecan
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens O75173
-
-

Purification (Commentary)

Purification (Comment) Organism
Macro S, gelatin-agarose, affinity purification with bovine tissue inhibitor of metalloproteinases and an aggrecanase-inhibitor resin Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain mRNA is present in brain tissue Homo sapiens
-
heart mRNA is present in heart tissue Homo sapiens
-
lung mRNA is present in lung tissue Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aggrecan + H2O
-
 Bos taurus fragments of aggrecan
-
 ?
aggrecan + H2O aggrecanase-1 does not cleave type II collagen, Tsp, fibronectin, casein or gelatin Homo sapiens fragments of aggrecan
-
 ?

Subunits

Subunits Comment Organism
?
-
 Homo sapiens
? x * 62000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
ADAMTS-4 [a disintegrin and metalloproteinase with thrombospondin motifs-4]
-
 Bos taurus
ADAMTS-4 [a disintegrin and metalloproteinase with thrombospondin motifs-4]
-
 Homo sapiens