Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.81 extracted from

  • Minond, D.; Cudic, M.; Bionda, N.; Giulianotti, M.; Maida, L.; Houghten, R.; Fields, G.
    Discovery of novel inhibitors of a disintegrin and metalloprotease 17 (ADAM17) using glycosylated and non-glycosylated substrates (2012), J. Biol. Chem., 287, 36473-36487.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.81

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0085
-
 Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser(glycosyl)-Ser-Lys(DABCYL) pH 7.5, 22°C Homo sapiens
0.012
-
 Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Lys(DABCYL) pH 7.5, 22°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O14672
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser(glycosyl)-Ser-Lys(DABCYL) + H2O
-
 Homo sapiens Glu(EDANS)-Pro-Leu-Ala-Gln-Ala + Val-Arg-Ser-Ser(glycosyl)-Ser-Lys(DABCYL)
-
 ?
Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Lys(DABCYL) + H2O
-
 Homo sapiens Glu(EDANS)-Pro-Leu-Ala-Gln-Ala + Val-Arg-Ser-Ser-Ser-Lys(DABCYL)
-
 ?
interleukin-6 receptor subunit alpha + H2O
-
 Homo sapiens ? cleavage occurs between residues LPVQ357-DSSV. Substrate shows N-linked glycosylation 7 residues apart from scissile bond ?
probetacellulin + H2O
-
 Homo sapiens ? cleavage occurs between residues CVVA31-DGNS. Substrate shows N-linked glycosylation 3 residues apart from scissile bond ?
proheparin-binding EGF-like growth factor + H2O
-
 Homo sapiens ? cleavage occurs between residues RKVR62-DLQE. Substrate shows O-linked glycosylation 13 residues apart from scissile bond ?
protransforming growth factor alpha + H2O
-
 Homo sapiens ? cleavage occurs between residues AAA39-VVSH. Substrate shows N-linked glycosylation 14 residues apart from scissile bond ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser(glycosyl)-Ser-Lys(DABCYL) pH 7.5, 22°C Homo sapiens
0.28
-
 Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Lys(DABCYL) pH 7.5, 22°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8
-
 Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser(glycosyl)-Ser-Lys(DABCYL) pH 7.5, 22°C Homo sapiens
25
-
 Glu(EDANS)-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Lys(DABCYL) pH 7.5, 22°C Homo sapiens