Literature summary for 3.4.24.81 extracted from

Parkin, E.; Harris, B.
A disintegrin and metalloproteinase (ADAM)-mediated ectodomain shedding of ADAM10 (2009), J. Neurochem., 108, 1464-1479.

Search for more literature for 3.4.24.81

Cloned(Commentary)

Cloned (Comment)	Organism
ADAM10 is expressed as a wild-type protein and as a FLAG-tagged ADAM10 fusion protein in which the tag is inserted immediately C-terminal to the proprotein convertase recognition sequence RKKR. Expression is carried out by stable transfection of a human neuroblastoma SH-SY5Y cell line. Internally tagged ADAM10 fusion protein is easily detected in Western blot analysis. Catalytic activity of wild-type and internally tagged protein is indistinguishable	Homo sapiens

Cloned (Comment)

Organism

ADAM10 is expressed as a wild-type protein and as a FLAG-tagged ADAM10 fusion protein in which the tag is inserted immediately C-terminal to the proprotein convertase recognition sequence RKKR. Expression is carried out by stable transfection of a human neuroblastoma SH-SY5Y cell line. Internally tagged ADAM10 fusion protein is easily detected in Western blot analysis. Catalytic activity of wild-type and internally tagged protein is indistinguishable

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA672	a truncated soluble construct of ADAM10 lacking the transmembrane and cytosolic domains (truncation after Glu680), although correctly post-translationally processed and catalytically active with respect to a synthetic peptide substrate, is incapable of shedding cell-associated amyloid precursor protein (APP)	Homo sapiens
additional information	a GPI-anchored form of ADAM10 lacking the cytosolic, transmembrane and a-helical juxtamembrane regions of the wild-type protein is shed in a similar manner. Mutant fusion construct consists of the first 652 residues of wild-type ADAM10 fused, via a two residue linker, to the 24 residue GPI anchor signal sequence of human carboxypeptidase M	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
GW4023	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	SDS-PAGE, mature form wild-type	Homo sapiens
98000	-	SDS-PAGE, immature form wild-type	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	ADAM10 itself is subject to ectodomain shedding via a mechanism which is inhibited by ADAM inhibitor (GW4023) and stimulated by phorbol ester treatment of cells. The treatment of cells with GW4023 causes a reciprocal accumulation of membrane-associated mature ADAM10 in both cell lysates and extracellular membrane vesicles. ADAM9 is, at least in part, responsible for the ectodomain shedding of ADAM10	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	catalytic activity of wild-type and internally FLAG-tagged fusion protein is indistinguishable	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
amyloid precursor protein + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
ADAM10	-	Homo sapiens