Application | Comment | Organism |
---|---|---|
additional information | glycolysation of MT1-MMP may protect against autolysis and thus stabilize ative MT1-MMP | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E240A | loss of MT1-MMP activity | Homo sapiens |
additional information | deletions delta Cat or point mutations CHO-1 and CHO-4, which preserve a relative electrophoretic mobility shift following desialylation similar to the wild type protein, moreover mutant CHO-4 is insensitive to sialidase A treatment, CHO-3 and CHO-4 mtuants are unable to effectively catalyze pro-MMP-2 activation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GM6001 | blocks conversion of active 50 kDa wild type MT1-MMP to a 37 kDa species, inhibits autolysis of both wild type and CHO-4 MT1-MMP in a dose-dependent manner and with a similar inhibitory profile | Homo sapiens | |
TIMP-2 | under in vivo conditions primary inhibitor of MT1-MMP, increases endocytosis of wild type MT1-MMP | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
active MT1-MMP species, SDS-PAGE | Homo sapiens |
64000 | - |
purified protein, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P50281 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity purification from COS-7 cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MDA-MB-231 cell | - |
Homo sapiens | - |
additional information | ovarian cancer cell lines DOV13, OVCA429, OVCA433 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-MMP-2 + H2O | - |
Homo sapiens | ? | - |
? | |
Type I collagen + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
membrane type 1-matrix metalloproteinase | - |
Homo sapiens |
MT1-MMP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | 0.01 | GM6001 | partial blockade of autolysis of both wild type and CHO-4 mutant MT1-MMP | Homo sapiens | |
0.1 | - |
GM6001 | complete blockade of autolysis of both wild type and CHO-4 mutant MT1-MMP | Homo sapiens |