Literature summary for 3.4.24.80 extracted from

Wu, Y.I.; Munshi, H.G.; Sen, R.; Snipas, S.J.; Salvesen, G.S.; Fridman, R.; Stack, M.S.
Glycosylation broadens the substrate profile of membrane type 1 matrix metalloproteinase (2004), J. Biol. Chem., 279, 8278-8289.

Search for more literature for 3.4.24.80

Application

Application	Comment	Organism
additional information	glycolysation of MT1-MMP may protect against autolysis and thus stabilize ative MT1-MMP	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in COS-7 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E240A	loss of MT1-MMP activity	Homo sapiens
additional information	deletions delta Cat or point mutations CHO-1 and CHO-4, which preserve a relative electrophoretic mobility shift following desialylation similar to the wild type protein, moreover mutant CHO-4 is insensitive to sialidase A treatment, CHO-3 and CHO-4 mtuants are unable to effectively catalyze pro-MMP-2 activation	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
GM6001	blocks conversion of active 50 kDa wild type MT1-MMP to a 37 kDa species, inhibits autolysis of both wild type and CHO-4 MT1-MMP in a dose-dependent manner and with a similar inhibitory profile	Homo sapiens
TIMP-2	under in vivo conditions primary inhibitor of MT1-MMP, increases endocytosis of wild type MT1-MMP	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	active MT1-MMP species, SDS-PAGE	Homo sapiens
64000	-	purified protein, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P50281	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity purification from COS-7 cells	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
MDA-MB-231 cell	-	Homo sapiens	-
additional information	ovarian cancer cell lines DOV13, OVCA429, OVCA433	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pro-MMP-2 + H2O	-	Homo sapiens	?	-	?
Type I collagen + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
membrane type 1-matrix metalloproteinase	-	Homo sapiens
MT1-MMP	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.001	0.01	GM6001	partial blockade of autolysis of both wild type and CHO-4 mutant MT1-MMP	Homo sapiens
0.1	-	GM6001	complete blockade of autolysis of both wild type and CHO-4 mutant MT1-MMP	Homo sapiens

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Release 2023.1 (January 2023)