Activating Compound | Comment | Organism | Structure |
---|---|---|---|
IGF-I | the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, the mechanism of IGF-modulated proteolysis of IGFBP-4 and -5 involves changes in both the recognition of substrate and the turnover rate, overview | Homo sapiens | |
IGF-II | the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, the mechanism of IGF-modulated proteolysis of IGFBP-4 and -5 involves changes in both the recognition of substrate and the turnover rate, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
IGF-I | the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, overview | Homo sapiens | |
IGF-II | the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
0.00031 | - |
IGFBP-5 | pH 7.5, 37°C, in absence of IGF | Homo sapiens | |
0.00041 | - |
IGFBP-5 | pH 7.5, 37°C, in presence of IGF-I | Homo sapiens | |
0.00045 | - |
IGFBP-4 | pH 7.5, 37°C, in presence of IGF-II | Homo sapiens | |
0.00064 | - |
IGFBP-5 | pH 7.5, 37°C, in presence of IGF-II | Homo sapiens | |
0.00163 | - |
IGFBP-4 | pH 7.5, 37°C, in presence of IGF-I | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Zn2+ | PAPP-A is a metzincin metalloproteinase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IGF-binding protein-4 + H2O | Homo sapiens | regulation of insulin-like growth factor bioavailability through cleavage of the inhibitory binding protein is an important mechanism for the control of growth and development of vertebrate cells | ? | - |
? | |
IGF-binding protein-5 + H2O | Homo sapiens | regulation of insulin-like growth factor bioavailability through cleavage of the inhibitory binding protein is an important mechanism for the control of growth and development of vertebrate cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Cleavage of the Met135-/-Lys bond in insulin-like growth factor binding protein (IGFBP)-4, and the Ser143-/-Lys bond in IGFBP-5 | the mechanism of IGF-modulated proteolysis of IGFBP-4 and -5 involves changes in both the recognition of substrate and the turnover rate | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IGF-binding protein-2 + H2O | i.e. IGFBP-2, low activity | Homo sapiens | ? | - |
? | |
IGF-binding protein-4 + H2O | i.e. IGFBP-4 | Homo sapiens | ? | - |
? | |
IGF-binding protein-4 + H2O | regulation of insulin-like growth factor bioavailability through cleavage of the inhibitory binding protein is an important mechanism for the control of growth and development of vertebrate cells | Homo sapiens | ? | - |
? | |
IGF-binding protein-5 + H2O | i.e. IGFBP-5 | Homo sapiens | ? | - |
? | |
IGF-binding protein-5 + H2O | regulation of insulin-like growth factor bioavailability through cleavage of the inhibitory binding protein is an important mechanism for the control of growth and development of vertebrate cells | Homo sapiens | ? | - |
? | |
IGFBP-4 + H2O | - |
Homo sapiens | ? | - |
? | |
IGFBP-5 + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAPP-A | - |
Homo sapiens |
pregnancy-associated plasma protein-A | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
IGFBP-5 | pH 7.5, 37°C, in presence of IGF-I | Homo sapiens | |
0.47 | - |
IGFBP-5 | pH 7.5, 37°C, in presence of IGF-II | Homo sapiens | |
0.64 | - |
IGFBP-4 | pH 7.5, 37°C, in presence of IGF-II | Homo sapiens | |
0.75 | - |
IGFBP-5 | pH 7.5, 37°C, in absence of IGF | Homo sapiens | |
0.89 | - |
IGFBP-4 | pH 7.5, 37°C, in presence of IGF-I | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |