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Literature summary for 3.4.24.79 extracted from

  • Gyrup, C.; Oxvig, C.
    Quantitative analysis of insulin-like growth factor-modulated proteolysis of insulin-like growth factor binding protein-4 and -5 by pregnancy-associated plasma protein-A (2007), Biochemistry, 46, 1972-1980.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
IGF-I the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, the mechanism of IGF-modulated proteolysis of IGFBP-4 and -5 involves changes in both the recognition of substrate and the turnover rate, overview Homo sapiens
IGF-II the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, the mechanism of IGF-modulated proteolysis of IGFBP-4 and -5 involves changes in both the recognition of substrate and the turnover rate, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
IGF-I the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, overview Homo sapiens
IGF-II the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Homo sapiens
0.00031
-
IGFBP-5 pH 7.5, 37°C, in absence of IGF Homo sapiens
0.00041
-
IGFBP-5 pH 7.5, 37°C, in presence of IGF-I Homo sapiens
0.00045
-
IGFBP-4 pH 7.5, 37°C, in presence of IGF-II Homo sapiens
0.00064
-
IGFBP-5 pH 7.5, 37°C, in presence of IGF-II Homo sapiens
0.00163
-
IGFBP-4 pH 7.5, 37°C, in presence of IGF-I Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Zn2+ PAPP-A is a metzincin metalloproteinase Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
IGF-binding protein-4 + H2O Homo sapiens regulation of insulin-like growth factor bioavailability through cleavage of the inhibitory binding protein is an important mechanism for the control of growth and development of vertebrate cells ?
-
?
IGF-binding protein-5 + H2O Homo sapiens regulation of insulin-like growth factor bioavailability through cleavage of the inhibitory binding protein is an important mechanism for the control of growth and development of vertebrate cells ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Cleavage of the Met135-/-Lys bond in insulin-like growth factor binding protein (IGFBP)-4, and the Ser143-/-Lys bond in IGFBP-5 the mechanism of IGF-modulated proteolysis of IGFBP-4 and -5 involves changes in both the recognition of substrate and the turnover rate Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IGF-binding protein-2 + H2O i.e. IGFBP-2, low activity Homo sapiens ?
-
?
IGF-binding protein-4 + H2O i.e. IGFBP-4 Homo sapiens ?
-
?
IGF-binding protein-4 + H2O regulation of insulin-like growth factor bioavailability through cleavage of the inhibitory binding protein is an important mechanism for the control of growth and development of vertebrate cells Homo sapiens ?
-
?
IGF-binding protein-5 + H2O i.e. IGFBP-5 Homo sapiens ?
-
?
IGF-binding protein-5 + H2O regulation of insulin-like growth factor bioavailability through cleavage of the inhibitory binding protein is an important mechanism for the control of growth and development of vertebrate cells Homo sapiens ?
-
?
IGFBP-4 + H2O
-
Homo sapiens ?
-
?
IGFBP-5 + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
PAPP-A
-
Homo sapiens
pregnancy-associated plasma protein-A
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
IGFBP-5 pH 7.5, 37°C, in presence of IGF-I Homo sapiens
0.47
-
IGFBP-5 pH 7.5, 37°C, in presence of IGF-II Homo sapiens
0.64
-
IGFBP-4 pH 7.5, 37°C, in presence of IGF-II Homo sapiens
0.75
-
IGFBP-5 pH 7.5, 37°C, in absence of IGF Homo sapiens
0.89
-
IGFBP-4 pH 7.5, 37°C, in presence of IGF-I Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens