Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, 73% inhibition, addition of 10 mM dithiothreitol restores activity to 100% | Clostridium perfringens | |
additional information | not inhibited by E-64, pepstatin, bestatin, EDTA, antipain, APMSF, leupeptin, aprotinin and SSI | Clostridium perfringens | |
p-chloromercuribenzenesulfonate | 1 mM, 39% inhibition, addition of 10 mM dithiothreitol restores activity to 100% | Clostridium perfringens | |
PMSF | 1 mM, 68% inhibition after 1 h | Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51442 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
52000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
55458 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
55585 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
57000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
60000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Superose 12, hydroxyapatite, DEAE-5PW | Clostridium perfringens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed coat | coat of dormant spores | Clostridium perfringens | - |
spore | - |
Clostridium perfringens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
139363 units, 1 unit is defined as a decrease in the OD600 of 0.1 per min | Clostridium perfringens |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
? | x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
72 h, 80% loss of activity in the absence of CHAPS | Clostridium perfringens |
55 | - |
40 min at pH 7.0, no loss of activity in the presence of 0.1% CHAPS | Clostridium perfringens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.5 | - |
Clostridium perfringens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
at least 2 month at 4°C in the presence of 0.1% CHAPS, no loss of activity | Clostridium perfringens |