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Literature summary for 3.4.24.78 extracted from

  • Dignam, S.S.; Setlow, P.
    Bacillus megaterium spore protease. Action of the enzyme on peptides containing the amino acid sequence cleaved by the enzyme in vivo (1980), J. Biol. Chem., 255, 8408-8412.
    View publication on PubMed
Search for more literature for 3.4.24.78

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 spore protein A spore protein A, Km lower than 0.014 mM Priestia megaterium
0.008
-
 spore protein B Km lower than 0.008 mM Priestia megaterium
0.4
-
 N-acetyl-Phe-Glu-Ile-Ala-Ser-Glu-Phe-Gly-Val-Asn-Leu-Gly-Pro-Asp-Ala-Thr-Ala-Arg pH 7.4, 37°C Priestia megaterium
20
-
 N-acetyl-Thr-Glu-Phe-Ala-Ser-Glu-Phe pH 7.4, 37°C Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
spore protein A + H2O Priestia megaterium specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein A
-
 ?
spore protein A + H2O Priestia megaterium QN 8155 specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein A
-
 ?
spore protein B + H2O Priestia megaterium specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein B
-
 ?
spore protein B + H2O Priestia megaterium QN 8155 specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein B
-
 ?
spore protein C + H2O Priestia megaterium specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein C
-
 ?
spore protein C + H2O Priestia megaterium QN 8155 specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein C
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Priestia megaterium QN 8155
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Priestia megaterium

Source Tissue

Source Tissue Comment Organism Textmining
spore
-
 Priestia megaterium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-Phe-Glu-Ile-Ala-Ser-Glu-Phe-Gly-Val-Asn-Leu-Gly-Pro-Asp-Ala-Thr-Ala-Arg + H2O
-
 Priestia megaterium N-acetyl-Phe-Glu + Ile-Ala-Ser-Glu-Phe-Gly-Val-Asn-Leu-Gly-Pro-Asp-Ala-Thr-Ala-Arg
-
 ?
N-acetyl-Thr-Glu-Phe-Ala + H2O
-
 Priestia megaterium N-acetyl-Thr-Glu + Phe-Ala
-
 ?
N-acetyl-Thr-Glu-Phe-Ala-Ser-Glu-Phe + H2O
-
 Priestia megaterium N-acetyl-Thr-Glu + Phe-Ala-Ser-Glu-Phe
-
 ?
spore protein A + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium fragments of spore protein A
-
 ?
spore protein A + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium QN 8155 fragments of spore protein A
-
 ?
spore protein B + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium fragments of spore protein B
-
 ?
spore protein B + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium QN 8155 fragments of spore protein B
-
 ?
spore protein C + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium fragments of spore protein C
-
 ?
spore protein C + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium QN 8155 fragments of spore protein C
-
 ?