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Literature summary for 3.4.24.75 extracted from

  • Chandra Ojha, S.; Imtong, C.; Meetum, K.; Sakdee, S.; Katzenmeier, G.; Angsuthanasombat, C.
    Purification and characterization of the antibacterial peptidase lysostaphin from Staphylococcus simulans Adverse influence of Zn2+ on bacteriolytic activity (2018), Protein Expr. Purif., 151, 106-112 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3)pLysS cells Staphylococcus simulans

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Staphylococcus simulans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Staphylococcus simulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methicillin-resistant Staphylococcus aureus cell wall + H2O Staphylococcus simulans
-
 ?
-
 ?
Staphylococcus aureus cell wall + H2O Staphylococcus simulans
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans P10547
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized-Ni2+ affinity column chromatography and Superdex S200 gel filtration Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methicillin-resistant Staphylococcus aureus cell wall + H2O
-
 Staphylococcus simulans ?
-
 ?
Staphylococcus aureus cell wall + H2O
-
 Staphylococcus simulans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, His6-tagged recombinant mature enzyme, SDS-PAGE Staphylococcus simulans
? x * 53000, His6-tagged recombinant preproenzyme, calculated from amino acid sequence Staphylococcus simulans

Synonyms

Synonyms Comment Organism
Lss
-
 Staphylococcus simulans