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Literature summary for 3.4.24.75 extracted from

  • Gruendling, A.; Missiakas, D.M.; Schneewind, O.
    Staphylococcus aureus mutants with increased lysostaphin resistance (2006), J. Bacteriol., 188, 6286-6297.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.75

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged lysostaphin comprising residues 1 to 154 in Escherichia coli strain BL21(DE3) Staphylococcus simulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Staphylococcus simulans
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus simulans Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that specifically binds to the cell wall envelope of Staphylococcus aureus and cleaves the pentaglycine cross bridges of peptidoglycan, thereby killing staphylococci, screening of a collection of Staphylococcus aureus strain Newman transposon mutants for lysostaphin resistance, transposon insertion in SAV2335 is herein named lyrA, i.e. lysostaphin resistance A, activity against diverse Staphylococcus aureus strains with MIC-values of 0.0005-0.002 mg/ml, overview ?
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 ?
additional information Staphylococcus simulans TNK1 Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that specifically binds to the cell wall envelope of Staphylococcus aureus and cleaves the pentaglycine cross bridges of peptidoglycan, thereby killing staphylococci, screening of a collection of Staphylococcus aureus strain Newman transposon mutants for lysostaphin resistance, transposon insertion in SAV2335 is herein named lyrA, i.e. lysostaphin resistance A, activity against diverse Staphylococcus aureus strains with MIC-values of 0.0005-0.002 mg/ml, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans
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Staphylococcus simulans TNK1
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged lysostaphin comprising residues 1 to 154 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that specifically binds to the cell wall envelope of Staphylococcus aureus and cleaves the pentaglycine cross bridges of peptidoglycan, thereby killing staphylococci, screening of a collection of Staphylococcus aureus strain Newman transposon mutants for lysostaphin resistance, transposon insertion in SAV2335 is herein named lyrA, i.e. lysostaphin resistance A, activity against diverse Staphylococcus aureus strains with MIC-values of 0.0005-0.002 mg/ml, overview Staphylococcus simulans ?
-
 ?
additional information Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that specifically binds to the cell wall envelope of Staphylococcus aureus and cleaves the pentaglycine cross bridges of peptidoglycan, thereby killing staphylococci, screening of a collection of Staphylococcus aureus strain Newman transposon mutants for lysostaphin resistance, transposon insertion in SAV2335 is herein named lyrA, i.e. lysostaphin resistance A, activity against diverse Staphylococcus aureus strains with MIC-values of 0.0005-0.002 mg/ml, overview Staphylococcus simulans TNK1 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 in vivo assay at Staphylococcus simulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in vivo assay at Staphylococcus simulans