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Literature summary for 3.4.24.75 extracted from
- Cytoplasmic expression of mature glycylglycine endopeptidase lysostaphin with an amino terminal hexa-histidine in a soluble and catalytically active form in Escherichia coli (2006), Protein Expr. Purif., 45, 206-215.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21(DE3) | Staphylococcus simulans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26900 | - |
calculated from the plot of mobility versus log molecular weight of standards | Staphylococcus simulans |
| 27000 | - |
SDS-PAGE, mature enzyme | Staphylococcus simulans |
| 27200 | - |
theoretically estimated value | Staphylococcus simulans |
| 29000 | - |
Sephacryl S-200 gel filtration, His6-fused lysostaphin | Staphylococcus simulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus simulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal affinity chromatography on Ni-NTA resin | Staphylococcus simulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
11900 units per mg, after 12 fold purification, 1 unit is defined as the amount of enzyme required to decrease the OD(A600) of a suspension of Staphylococcus aureus 237 cells by 0.001 at 37°C in 5 min in a 3 ml reaction volume | Staphylococcus simulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Peptidoglycan + H2O | - |
Staphylococcus simulans | Fragments of peptidoglycan | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glycylglycine endopeptidase | - |
Staphylococcus simulans |
| r-lysostaphin | - |
Staphylococcus simulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | - |
Staphylococcus simulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | stable for 24 hours at pH 8.0 | Staphylococcus simulans |
| 40 | - |
stable for 4h with 80% activity | Staphylococcus simulans |
| 50 | - |
when incubated at temperatures from 25 to 70°C the enzyme is stable up to 50°C, above the activity is rapidly lost | Staphylococcus simulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Staphylococcus simulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Staphylococcus simulans |
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Release 2023.1 (January 2023)
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