Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.75 extracted from

  • Warfield, R.; Bardelang, P.; Saunders, H.; Chan, W.C.; Penfold, C.; James, R.; Thomas, N.R.
    Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus (2006), Org. Biomol. Chem., 4, 3626-3638.
    View publication on PubMed
Search for more literature for 3.4.24.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Staphylococcus simulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 L-lysine(N-e-pentaglycine-N-(2-aminobenzoyl))-[N-(2,4-dinitrophenyl)ethylenediamine] value from Grafit and Hanes plot analyses Staphylococcus simulans
0.07
-
 L-lysine(N-e-pentaglycyl-DABCYL)-EDANS value from Hanes plot analysis Staphylococcus simulans
0.08
-
 L-lysine(N-e-pentaglycyl-DABCYL)-EDANS value from Grafit plot analysis Staphylococcus simulans
0.2
-
 N-(2-Aminobenzoyl)pentaglycine-[N-(2,4-dinitrophenyl)ethylenediamine] value from Grafit plot analysis Staphylococcus simulans
0.3
-
 N-(2-Aminobenzoyl)pentaglycine-[N-(2,4-dinitrophenyl)ethylenediamine] value from Hanes plot analysis Staphylococcus simulans

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans
-
 biovar Staphylococcus simulans staphylolyticus
-

Purification (Commentary)

Purification (Comment) Organism
metal-affinity chromatography and cation exchange chromatography Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine(N-e-pentaglycine-N-(2-aminobenzoyl))-[N-(2,4-dinitrophenyl)ethylenediamine]
-
 Staphylococcus simulans ?
-
 ?
L-lysine(N-e-pentaglycyl-DABCYL)-EDANS + H2O
-
 Staphylococcus simulans ?
-
 ?
L-lysine(N-epsilon-pentaglycine-N-(2-aminobenzoyl))-[N-(2,4-dinitrophenyl)ethylenediamine] + H2O
-
 Staphylococcus simulans ?
-
 ?
L-lysine(N-epsilon-pentaglycyl-DABCYL)-EDANS + H2O
-
 Staphylococcus simulans ?
-
 ?
N-(2-aminobenzoyl)pentaglycine-[N-(2,4-dinitrophenyl)ethylenediamine] + H2O
-
 Staphylococcus simulans ?
-
 ?