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Literature summary for 3.4.24.75 extracted from

  • Szweda, P.; Kotlowski, R.; Kur, J.
    New effective sources of the Staphylococcus simulans lysostaphin (2005), J. Biotechnol., 117, 203-213.
    View publication on PubMed
Search for more literature for 3.4.24.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus simulans

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 10 mM, 30% decrease of activity of enzyme produced with pET vector system and 20% decrease of activity of enzyme produced with pBAD vector system Staphylococcus simulans
Cu2+ 2 mM, 70-80% decrease of activity Staphylococcus simulans
additional information no inhibitory effect of EDTA and 2 mM Co2+ Staphylococcus simulans
Ni2+ 2 mM, 60% decrease of activity of enzyme produced with pET vector system and 20% decrease of activity of enzyme produced with pBAD vector system Staphylococcus simulans
Zn2+ 2 mM, 70-80% decrease of activity Staphylococcus simulans

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans P10547
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt Sepharose column chromatography Staphylococcus simulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 970 U/mg Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Peptidoglycan + H2O
-
 Staphylococcus simulans Fragments of peptidoglycan
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Staphylococcus simulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 enzymeproduced with pBAD vector system Staphylococcus simulans
8
-
 enzyme obtained from pET vector system Staphylococcus simulans