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Literature summary for 3.4.24.74 extracted from

  • Wu, S.; Shin, J.; Zhang, G.; Cohen, M.; Franco, A.; Sears, C.L.
    The Bacteroides fragilis toxin binds to a specific intestinal epithelial cell receptor (2006), Infect. Immun., 74, 5382-5390.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information intestinal epithelial cells in vitro possess a specific membrane BFT receptor that is distinct from E-cadherin Bacteroides fragilis

Protein Variants

Protein Variants Comment Organism
H352Y Alexa-488-labeled BFT binding to HT29/C1 not blocked Bacteroides fragilis
additional information BFT-C4, which has reduced BFT biological activity, shows a significant decrease in HT29/C1 cell binding Bacteroides fragilis

Inhibitors

Inhibitors Comment Organism Structure
germ agglutinin inhibits BFT biological activity on HT29/C1 cells but not binding of Alexa 488-labeled BFT to HT29/C1 cells Bacteroides fragilis
GM6001 significantly reduces Alexa 488-labeled BFT binding Bacteroides fragilis
additional information not inhibited by lectins, sugars or mixed bovine brain gangliosides Bacteroides fragilis
subtilisin A inhibits both BFT biological activity on HT29/C1 cells and nearly 90% of binding of Alexa 488-labeled BFT Bacteroides fragilis
Thermolysin inhibits both BFT biological activity on HT29/C1 cells and nearly 90% of binding of Alexa 488-labeled BFT Bacteroides fragilis

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-
Bacteroides fragilis I-1345
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity by urea-based purification method Bacteroides fragilis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
E-cadherin + H2O neither human nor rat E-cadherin are the cellular receptor for BFT Bacteroides fragilis ?
-
?
E-cadherin + H2O neither human nor rat E-cadherin are the cellular receptor for BFT Bacteroides fragilis I-1345 ?
-
?

Synonyms

Synonyms Comment Organism
Bacteroides fragilis toxin
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Bacteroides fragilis
BFT
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Bacteroides fragilis