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Literature summary for 3.4.24.73 extracted from

  • Garcia, L.T.; Parreiras e Silva, L.T.; Ramos, O.H.; Carmona, A.K.; Bersanetti, P.A.; Selistre-de-Araujo, H.S.
    The effect of post-translational modifications on the hemorrhagic activity of snake venom metalloproteinases (2004), Comp. Biochem. Physiol. C, 138, 23-32.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Bothrops jararaca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0051
-
Abz-LVEALYQ-EDDnp native jararhagin Bothrops jararaca
0.0078
-
Abz-LVEALYQ-EDDnp deglycosylated jararhagin Bothrops jararaca
0.0297
-
Abz-LVEALYQ-EDDnp catalytic domain of jararhagin Bothrops jararaca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
SDS-PAGE, catalytic domain of jararhagin Bothrops jararaca
52000
-
SDS-PAGE, jararhagin Bothrops jararaca

Organism

Organism UniProt Comment Textmining
Bothrops jararaca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography Bothrops jararaca

Renatured (Commentary)

Renatured (Comment) Organism
refolding after treatment with 6 M urea Bothrops jararaca

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Bothrops jararaca
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-LVEALYQ-EDDnp + H2O
-
Bothrops jararaca ?
-
?
Fibrin + H2O
-
Bothrops jararaca ?
-
?
Fibrinogen + H2O
-
Bothrops jararaca ?
-
?
Fibronectin + H2O
-
Bothrops jararaca ?
-
?

Synonyms

Synonyms Comment Organism
hemorrhagic svMP
-
Bothrops jararaca
rCDJARA catalytic domain of jararhagin Bothrops jararaca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0105
-
Abz-LVEALYQ-EDDnp catalytic domain of jararhagin Bothrops jararaca
0.093
-
Abz-LVEALYQ-EDDnp native jararhagin Bothrops jararaca
0.14
-
Abz-LVEALYQ-EDDnp deglycosylated jararhagin Bothrops jararaca