Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 95.08% residual activity at 10 mM | Bacillus amyloliquefaciens | |
Al3+ | 3.5% residual activity at 10 mM | Bacillus amyloliquefaciens | |
Ba2+ | 79.86% residual activity at 10 mM | Bacillus amyloliquefaciens | |
Ca2+ | 87.04% residual activity at 10 mM | Bacillus amyloliquefaciens | |
Cs2+ | 89.14% residual activity at 10 mM | Bacillus amyloliquefaciens | |
Cu2+ | 4.55% residual activity at 10 mM | Bacillus amyloliquefaciens | |
dithiothreitol | 84.92% residual activity at 10 mM | Bacillus amyloliquefaciens | |
EDTA | 2.15% residual activity at 10 mM | Bacillus amyloliquefaciens | |
iodoacetic acid | 80.54% residual activity at 10 mM | Bacillus amyloliquefaciens | |
K+ | 86.69% residual activity at 10 mM | Bacillus amyloliquefaciens | |
Mg2+ | 92.64% residual activity at 10 mM | Bacillus amyloliquefaciens | |
Na+ | 84.41% residual activity at 10 mM | Bacillus amyloliquefaciens | |
pepstatin A | 84.31% residual activity at 10 mM | Bacillus amyloliquefaciens | |
phenylmethylsulfonyl fluoride | 8.62% residual activity at 10 mM | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the activity of the enzyme is enhanced to 111.21% in the presence of 10 mM Mn2+ | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus amyloliquefaciens MCC2606 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose ion-exchange column chromatography and Sephadex G-75 gel filtration | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | 16.62% activity compared to human fibrin | Bacillus amyloliquefaciens | ? | - |
? | |
Bovine serum albumin + H2O | 16.62% activity compared to human fibrin | Bacillus amyloliquefaciens MCC2606 | ? | - |
? | |
casein + H2O | 60.36% activity compared to human fibrin | Bacillus amyloliquefaciens | ? | - |
? | |
casein + H2O | 60.36% activity compared to human fibrin | Bacillus amyloliquefaciens MCC2606 | ? | - |
? | |
fibrinogen + H2O | 55.21% activity compared to human fibrin. Among the three chains of fibrinogen, Aalpha and Bbeta chains are degraded rapidly, whereas gamma-chain of fibrinogen shows significant resistance to degradation by the enzyme | Bacillus amyloliquefaciens | fibrin + ? | - |
? | |
fibrinogen + H2O | 55.21% activity compared to human fibrin. Among the three chains of fibrinogen, Aalpha and Bbeta chains are degraded rapidly, whereas gamma-chain of fibrinogen shows significant resistance to degradation by the enzyme | Bacillus amyloliquefaciens MCC2606 | fibrin + ? | - |
? | |
Hemoglobin + H2O | 25.09% activity compared to human fibrin | Bacillus amyloliquefaciens | ? | - |
? | |
Hemoglobin + H2O | 25.09% activity compared to human fibrin | Bacillus amyloliquefaciens MCC2606 | ? | - |
? | |
human fibrin + H2O | fibrin is the most preferred substrate (100% activity). The beta-chain of fibrin is the primary component and site susceptible for the enzyme in early action. The enzyme can completely degrade all the four chains of fibrin | Bacillus amyloliquefaciens | ? | - |
? | |
human fibrin + H2O | fibrin is the most preferred substrate (100% activity). The beta-chain of fibrin is the primary component and site susceptible for the enzyme in early action. The enzyme can completely degrade all the four chains of fibrin | Bacillus amyloliquefaciens MCC2606 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Bacillus amyloliquefaciens |
Synonyms | Comment | Organism |
---|---|---|
CFR15-protease | - |
Bacillus amyloliquefaciens |
fibrinolytic protease | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus amyloliquefaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme is active between 30 and 60°C | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 11 | the enzyme is active between pH 5.5 and 11.0 | Bacillus amyloliquefaciens |