Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 29% residual activity at 5 mM | Urechis unicinctus | |
Cu2+ | 32% residual activity at 5 mM | Urechis unicinctus | |
EDTA | 45% residual activity at 1 mM | Urechis unicinctus | |
EGTA | 36% residual activity at 1 mM | Urechis unicinctus | |
Fe2+ | 70% residual activity at 5 mM | Urechis unicinctus | |
Fe3+ | 36% residual activity at 5 mM | Urechis unicinctus | |
phenylmethylsulfonyl fluoride | 28% residual activity at 4 mM | Urechis unicinctus | |
protein SBTI | 26% residual activity at 4 mM | Urechis unicinctus | |
Zn2+ | 42% residual activity at 5 mM | Urechis unicinctus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 118% activity at 5 mM | Urechis unicinctus | |
Mn2+ | 118% activity at 5 mM | Urechis unicinctus | |
additional information | not influenced by Ca2+ | Urechis unicinctus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20800 | - |
1 * 20800, SDS-PAGE | Urechis unicinctus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibrinogen + H2O | Urechis unicinctus | - |
fibrin + ? | - |
? | |
plasminogen + H2O | Urechis unicinctus | - |
plasmin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Urechis unicinctus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Sephacryl S-100 gel filtration, and Sephadex G-50 gel filtration | Urechis unicinctus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Urechis unicinctus | ? | - |
? | |
fibrinogen + H2O | - |
Urechis unicinctus | fibrin + ? | - |
? | |
additional information | the enzyme was devoid of hemorrhagic effect | Urechis unicinctus | ? | - |
? | |
plasminogen + H2O | - |
Urechis unicinctus | plasmin + ? | - |
? | |
S-2444 + H2O | i.e. pyroGlu-Gly-Arg-4-nitroanilide | Urechis unicinctus | pyroGlu-Gly-Arg + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 20800, SDS-PAGE | Urechis unicinctus |
Synonyms | Comment | Organism |
---|---|---|
fibrinolytic protease | - |
Urechis unicinctus |
UFEIII | - |
Urechis unicinctus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | - |
Urechis unicinctus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme remains stable between 30 and 60°C when incubated for 40 min | Urechis unicinctus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
at approximately 55°C | Urechis unicinctus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | more than 50% activity between pH 6.0 and 10.0 | Urechis unicinctus |