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Literature summary for 3.4.24.72 extracted from

  • Swenson, S.; Markland, F.S.
    Fibrolase (2006), Toxin Rev., 25, 351-378.
No PubMed abstract available
Search for more literature for 3.4.24.72

Crystallization (Commentary)

Crystallization (Comment) Organism
structure analysis Agkistrodon contortrix contortrix

Protein Variants

Protein Variants Comment Organism
additional information construction of a heterobifunctional chimeric derivative of fibrolase through linking covalently with a RGD-like peptide via a surface residue distant from the active site, structure, overview, the chimera shows fibrolase and platelet-binding ability, the chimeric enzyme exhibits an IC50 of 105 nM in inhibition of platelet aggregation, overview Agkistrodon contortrix contortrix

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline rapid and complete inhibition Agkistrodon contortrix contortrix
alpha2-Macroglobulin the 720 kDa tetrameric inhibitor causes rapid and irreversible inhibition by sterical hindrance, the PEG-bound enzyme is less sensitive Agkistrodon contortrix contortrix
EDTA rapid and complete inhibition Agkistrodon contortrix contortrix
additional information polyethylene glycol addition to the enzyme modifies the enzyme size and reduces its activity Agkistrodon contortrix contortrix
tetraethylene pentamine rapid and complete inhibition Agkistrodon contortrix contortrix

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Agkistrodon contortrix contortrix
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 1 mol per mol of enzyme, fibrolase is a zinc metalloprotease with the zinc binding motif HEXXHXXGXXH Agkistrodon contortrix contortrix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 x * 23000, native enzyme, x * 60000, PEG-bound enzyme Agkistrodon contortrix contortrix
43330
-
 PEG-bound enzyme, gel filtration Agkistrodon contortrix contortrix
60000
-
 x * 23000, native enzyme, x * 60000, PEG-bound enzyme Agkistrodon contortrix contortrix

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fibrin + H2O Agkistrodon contortrix contortrix fibrinolytic activity ?
-
 ?
fibrinogen + H2O Agkistrodon contortrix contortrix fibrinogenolytic activity fibrin + propeptide
-
 ?
low-molecular weight kininogen + H2O Agkistrodon contortrix contortrix initiates generation of bradykinin kallidin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Agkistrodon contortrix contortrix
-
 southern copperhead snake
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by hydrophobic interaction and hydroxyapatite chromatography Agkistrodon contortrix contortrix

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Agkistrodon contortrix contortrix
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1200
-
 purified PEG-bound enzyme, substrate azocasein Agkistrodon contortrix contortrix
1260
-
 purified native enzyme, substrate azocasein Agkistrodon contortrix contortrix
10900
-
 purified PEG-bound enzyme, substrate human fibrin Agkistrodon contortrix contortrix
11300
-
 purified native enzyme, substrate human fibrin Agkistrodon contortrix contortrix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-amino-benzene-His-Thr-Glu-Lys-leu-Val-Thr-Ser-dinitrophenol + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
azocasein + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
Fibrin + H2O fibrinolytic activity Agkistrodon contortrix contortrix ?
-
 ?
Fibrin + H2O human substrate, fibrinolytic activity cleaving the Lys413-Leu414 bond Agkistrodon contortrix contortrix ?
-
 ?
fibrinogen + H2O fibrinogenolytic activity Agkistrodon contortrix contortrix fibrin + propeptide
-
 ?
His-Thr-Glu-Ala-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-Arg-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-Asn-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-Glu-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-His-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-Leu-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-Lys-Leu-Val-Thr-Ser + H2O cleaving sequence residues 410-417 from fibrinogen Aalpha-chain Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-Phe-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-Pro-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
His-Thr-Glu-Ser-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix contortrix ?
-
 ?
Insulin B-chain + H2O fibrinogenolytic activity cleaving the Leu11-Val-Glu-Ala-Leu-Tyr-Leu-Val18 peptide Agkistrodon contortrix contortrix ?
-
 ?
Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val + H2O cleaving sequence residues 11-18 from insulin B-chain Agkistrodon contortrix contortrix ?
-
 ?
low-molecular weight kininogen + H2O
-
 Agkistrodon contortrix contortrix kallidin + ?
-
 ?
low-molecular weight kininogen + H2O initiates generation of bradykinin Agkistrodon contortrix contortrix kallidin + ?
-
 ?
additional information the enzyme is a nonhemorrhagic zinc metalloprotease, peptide substrate specificity, preference for Xaa-Leu and Xaa-Phe overview Agkistrodon contortrix contortrix ?
-
 ?

Subunits

Subunits Comment Organism
? x * 23000, native enzyme, x * 60000, PEG-bound enzyme Agkistrodon contortrix contortrix
More the protein is composed of 5 alpha-helical regions, 4 parallel and 1 antiparallel beta-sheet, stablized by 3 disulfide bonds Agkistrodon contortrix contortrix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Agkistrodon contortrix contortrix

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information native and PEG-bound enzyme, rapid interaction kinetics with inhibitor alpha2-macroglobulin Agkistrodon contortrix contortrix

pI Value

Organism Comment pI Value Maximum pI Value
Agkistrodon contortrix contortrix
-
-
 6.8