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Literature summary for 3.4.24.72 extracted from
- Purification and characterization of a fibrinolytic enzyme from venom of the southern copperhead snake (Agkistrodon contortrix contortrix) (1991), Arch. Biochem. Biophys., 289, 197-207.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Agkistrodon contortrix contortrix |  |
| EDTA | - |
Agkistrodon contortrix contortrix | |
| additional information | not: DFP, soybean trypsin inhibitor, trasylol, PCMB | Agkistrodon contortrix contortrix | |
| Tetraethylenepentamine | - |
Agkistrodon contortrix contortrix | |
| Zincov | - |
Agkistrodon contortrix contortrix | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | zinc metalloproteinase | Agkistrodon contortrix contortrix | |
| Zinc | enzyme contains 1 mol of zinc per mol of protein | Agkistrodon contortrix contortrix | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22540 | - |
Agkistrodon contortrix, calculation from amino acid sequence | Agkistrodon contortrix contortrix |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agkistrodon contortrix contortrix | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | contains little or no carbohydrate | Agkistrodon contortrix contortrix |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Agkistrodon contortrix contortrix |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Agkistrodon contortrix contortrix | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Agkistrodon contortrix contortrix |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, stable for 6 months, 20-30% loss of activity after 1 year | Agkistrodon contortrix contortrix |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Agkistrodon contortrix contortrix | ? | - |
? | |
| Insulin B-chain + H2O | cleavage between Ala14 and Leu15 | Agkistrodon contortrix contortrix | ? | - |
? | |
| additional information | no activity against a series of chromogenic 4-nitroanilide substrates | Agkistrodon contortrix contortrix | ? | - |
? | |
| additional information | exhibits little if any hemorrhagic activity | Agkistrodon contortrix contortrix | ? | - |
? | |
| additional information | does not activate plasminogen | Agkistrodon contortrix contortrix | ? | - |
? | |
| additional information | direct fibrinolytic activity | Agkistrodon contortrix contortrix | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000-24000, Agkistrodon contortrix, SDS-PAGE under reducing and nonreducing condition | Agkistrodon contortrix contortrix |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
6 h, 30-35% loss of activity | Agkistrodon contortrix contortrix |
| 6.5 | - |
optimal pH for stability | Agkistrodon contortrix contortrix |
| 9 | - |
6 h, 45-50% loss of activity | Agkistrodon contortrix contortrix |
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