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Literature summary for 3.4.24.71 extracted from

  • Schulz, H.; Dale, G.E.; Karimi-Nejad, Y.; Oefner, C.
    Structure of human endothelin-converting enzyme I complexed with phosphoramidon (2009), J. Mol. Biol., 385, 178-187.
    View publication on PubMed
Search for more literature for 3.4.24.71

Cloned(Commentary)

Cloned (Comment) Organism
extracellular domain of monomeric human ECE-1 (C428S) mutant (Q90-W770) is amplified by PCR and produced recombinantly in Escherichia coli (inclusion bodies) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
cocrystallization of C428S mutant of monomeric form (extracellular domain, residues 90-770) with inhibitor N-(alpha-L-rhamnopyranosyl-oxyhydroxy-phosphinyl)-L-Leu-L-Trp Homo sapiens

Protein Variants

Protein Variants Comment Organism
C428S mutant of monomeric form Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-(alpha-L-rhamnopyranosyl-oxyhydroxy-phosphinyl)-L-Leu-L-Trp mechanism of inhibitor binding analysed (cocrystallization) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ binding site is located in the C-terminal domain and is close to the inner surface of the protein, coordinated in an approximately tetrahedral geometry, involving the side chains of the residues His607, His611, and Glu667 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 crystallization, 680 residues of monomeric enzyme (extracellular domain, residues 90-770) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
big endothelin-1 + H2O Homo sapiens
-
 endothelin-1 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P42892
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information intra-molecular disulfide bond formed by C428 (homodimer) Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
cell rupture by lysozyme and continuous cell disrupter, washing of insoluble pellet Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
inclusion bodies solubilized in Tris-HCl, urea, beta-mercaptoethanol, dithiotreithol, glutathione, glycine Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
big endothelin-1 + H2O
-
 Homo sapiens endothelin-1 + ?
-
 ?

Subunits

Subunits Comment Organism
homodimer forms in vivo, intra-molecular disulfide bond formed by C428, here crystallisation of monomeric mutant (extracellular domain, residues 90-770) Homo sapiens

Synonyms

Synonyms Comment Organism
ECE-1
-
 Homo sapiens
endothelin-converting enzyme I
-
 Homo sapiens