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Literature summary for 3.4.24.71 extracted from
- Purification and characterization of endothelin-converting enzyme from rat lung (1993), J. Biol. Chem., 268, 21394-21398.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Rattus norvegicus |  |
| EDTA | - |
Rattus norvegicus | |
| additional information | no inhibition by thiorphan, captopril | Rattus norvegicus | |
| phosphoramidon | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
Big endothelin-1 | - |
Rattus norvegicus | |
| 0.00027 | - |
big endothelin-3 | - |
Rattus norvegicus | |
| 0.00046 | - |
big endothelin-2 | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | neutral metalloendopeptidase | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
1 * 130000, SDS-PAGE | Rattus norvegicus |
| 170000 | 190000 | rat, FPLC gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Big endothelin 1 + H2O | Rattus norvegicus | key enzyme in endothelin production, generates potent vasoconstrictor endothelin from its inactive precursor | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lung | lung enzyme is similar to that of endothelial cells | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00127 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Big endothelin 1 + H2O | key enzyme in endothelin production, generates potent vasoconstrictor endothelin from its inactive precursor | Rattus norvegicus | ? | - |
? | |
| big endothelin-2 + H2O | - |
Rattus norvegicus | ? | - |
? | |
| big endothelin-3 + H2O | - |
Rattus norvegicus | endothelin-3 + ? | - |
? | |
| Human big endothelin 1 + H2O | best substrate | Rattus norvegicus | Endothelin 1 + big endothelin 1(21-38) | - |
? | |
| Human big endothelin 2 + H2O | hydrolysis at 25% the rate of big endothelin1 | Rattus norvegicus | ? | - |
? | |
| Human big endothelin 3 + H2O | hydrolysis at 50% the rate of big endothelin1 | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 130000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 6.8 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.2 | active in a narrow range | Rattus norvegicus |
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