Cloned (Comment) | Organism |
---|---|
N-terminally His-tagged isozyme overexpression in Escherichia coli BL21 Rosetta (DE3) | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
purified enzyme TOP2 bound to 4-azido-salicylate, sitting-drop vapor-diffusion method, mixing of 40 mg/ml protein in 20 mM Tris, pH 8.5, 200 mM NaCl, 5 mM DTT, 5% v/v glycerol, with reservoir solution consisting of 220 mM ammonium acetate, 20% w/v PEG 3350, 20°C, X-ray diffraction structure determination and analysis at 3.0 A resolution | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
salicylic acid | inhibits the peptidase activity of isozyme TOP1 and to a lesser extent isozyme TOP2; inhibits the peptidase activity of isozyme TOP1 and to a lesser extent isozyme TOP2 | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc-dependent metallopeptidase | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q949P2 | isozyme TOP2; isozyme TOP2 | - |
Arabidopsis thaliana | Q94AM1 | isozyme TOP1; isozyme TOP1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme from Escherichia coli BL21 Rosetta (DE3) by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
TOP | - |
Arabidopsis thaliana |
Top1 | - |
Arabidopsis thaliana |
Top2 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the TOP isozymes contain the conserved His-Glu-XX-His (HEXXH) sequence of active-site residues and belong to the M3 family of metallopeptidases | Arabidopsis thaliana |
physiological function | the enzyme is a target for salicylic acid binding and participate in SA-mediated plant innate immunity. Both isozymes TOP1 and TOP2 seem to play a role in salicylic acid-dependent innate immunity, but through independent pathways | Arabidopsis thaliana |