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Literature summary for 3.4.24.70 extracted from
- Support for a potential role of E. coli oligopeptidase A in protein degradation (2007), Biochem. Biophys. Res. Commun., 359, 486-490.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene opdA, DNA and amino acid sequence determination and anaylsis, expression of the His-tagged enzyme in strain BL21(DE3) | Escherichia coli K-12 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli K-12 |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli K-12 | the enzyme digests proteins to short peptides that are subsequently hydrolyzed to smaller fragments and free amino acids, protein degradation is an essential quality control and regulatory function | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli K-12 | - |
gene opdA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography | Escherichia coli K-12 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-casein-derived peptides + H2O | alpha-casein is not cleaved by OpdA, but by proteases HslUV, ClpAP, or Lon, the peptide products are then cleaved by OpdA, overview | Escherichia coli K-12 | ? | - |
? | |
| additional information | the enzyme digests proteins to short peptides that are subsequently hydrolyzed to smaller fragments and free amino acids, protein degradation is an essential quality control and regulatory function | Escherichia coli K-12 | ? | - |
? | |
| additional information | the enzyme cleaves peptides generated by the activity of the three primary ATP-dependent proteases Lon, HslUV, and ClpAP from Escherichia coli, overview | Escherichia coli K-12 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| oligopeptidase A | - |
Escherichia coli K-12 |
| OpdA | - |
Escherichia coli K-12 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Escherichia coli K-12 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Escherichia coli K-12 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | ATP-dependent protease | Escherichia coli K-12 | |
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Release 2023.1 (January 2023)
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