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Literature summary for 3.4.24.70 extracted from

  • Paschoalin, T.; Carmona, A.K.; Oliveira, V.; Juliano, L.; Travassos, L.R.
    Characterization of thimet- and neurolysin-like activities in Escherichia coli M3A peptidases and description of a specific substrate (2005), Arch. Biochem. Biophys., 441, 25-34.
    View publication on PubMed
Search for more literature for 3.4.24.70

Cloned(Commentary)

Cloned (Comment) Organism
expressed from pHis3-OpdA Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline totally inhibits at 4 mM Escherichia coli
JA-2 totally inhibits at 0.003 mM Escherichia coli
additional information not inhibited by 0.2 mM leupeptin, 0.1 mM PMSF, 0.1 mM E-64, 0.001 mM Z-pro-prolinal and 0.02 mM captopril Escherichia coli
Pro-Ile partly inhibits Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77100
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli BL21 (DE3) pLysS
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by nickel affinity chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0662
-
 with o-aminobenzoyl-GFSPFRQ-N-(2,4-dinitrophenyl)-ethylenediamine as substrate Escherichia coli
0.0833
-
 with o-aminobenzoyl-GFSPFR-N-(2,4-dinitrophenyl)-ethylenediamine as substrate Escherichia coli
0.0858
-
 with o-aminobenzoyl-RPPGFSPFRQ-N-(2,4-dinitrophenyl)-ethylenediamine as substrate Escherichia coli
0.1129
-
 with o-aminobenzoyl-GFSIFRQ-N-(2,4-dinitrophenyl)-ethylenediamine as substrate Escherichia coli
0.289
-
 with o-aminobenzoyl-NKPRRPQ-N-(2,4-dinitrophenyl)-ethylenediamine as substrate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bradykinin + H2O
-
 Escherichia coli ?
-
 ?
bradykinin + H2O
-
 Escherichia coli BL21 (DE3) pLysS ?
-
 ?
additional information no cleavage of Abz-GFPPFRQ-N-(2,4-dinitrophenyl)-ethylenediamine Escherichia coli ?
-
 ?
additional information no cleavage of Abz-GFPPFRQ-N-(2,4-dinitrophenyl)-ethylenediamine Escherichia coli BL21 (DE3) pLysS ?
-
 ?
neurotensin + H2O
-
 Escherichia coli ?
-
 ?
neurotensin + H2O
-
 Escherichia coli BL21 (DE3) pLysS ?
-
 ?
o-aminobenzoyl-GFSIFRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Escherichia coli ?
-
 ?
o-aminobenzoyl-GFSIFRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Escherichia coli BL21 (DE3) pLysS ?
-
 ?
o-aminobenzoyl-GFSPFR-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Escherichia coli ?
-
 ?
o-aminobenzoyl-GFSPFR-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Escherichia coli BL21 (DE3) pLysS ?
-
 ?
o-aminobenzoyl-GFSPFRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Escherichia coli ?
-
 ?
o-aminobenzoyl-NKPRRPQ-N-(2,4-dinitrophenyl)-ethylenediamine best substrate for OpdA Escherichia coli ?
-
 ?
o-aminobenzoyl-RPPGFSPFRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
OpdA
-
 Escherichia coli