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Literature summary for 3.4.24.69 extracted from

  • Sikorra, S.; Skiba, M.; Dorner, M.B.; Weisemann, J.; Weil, M.; Valdezate, S.; Davletov, B.; Rummel, A.; Dorner, B.G.; Binz, T.
    Botulinum neurotoxin F subtypes cleaving the VAMP-2 Q58-K59 peptide bond exhibit unique catalytic properties and substrate specificities (2018), Toxins, 10, E311 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15pREP4 cells Clostridium botulinum
expressed in Escherichia coli M15pREP4 cells Clostridium baratii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0287
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F1, at pH 6.8 and 37°C Clostridium botulinum
0.0441
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F9, at pH 6.8 and 37°C Clostridium botulinum
0.0701
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F6, at pH 6.8 and 37°C Clostridium botulinum
0.0753
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F7, at pH 6.8 and 37°C Clostridium baratii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vesicle-associated membrane protein-2 + H2O Clostridium botulinum cleavage at Gln58-Lys59 ?
-
 ?
vesicle-associated membrane protein-2 + H2O Clostridium baratii cleavage at Gln58-Lys59 ?
-
 ?
vesicle-associated membrane protein-2 + H2O Clostridium baratii CNM1212/11 cleavage at Gln58-Lys59 ?
-
 ?
vesicle-associated membrane protein-2 + H2O Clostridium botulinum NCTC 10281 cleavage at Gln58-Lys59 ?
-
 ?
vesicle-associated membrane protein-2 + H2O Clostridium botulinum H078-01 cleavage at Gln58-Lys59 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium baratii A0A1P8YWP1
-
-
Clostridium baratii CNM1212/11 A0A1P8YWP1
-
-
Clostridium botulinum A0A1P8YWK9
-
-
Clostridium botulinum A7GBG3
-
-
Clostridium botulinum D2KHS4
-
-
Clostridium botulinum H078-01 A0A1P8YWK9
-
-
Clostridium botulinum H078-01 D2KHS4
-
-
Clostridium botulinum NCTC 10281 A7GBG3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose bead chromatography Clostridium botulinum
Ni-NTA agarose bead chromatography Clostridium baratii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADAL + H2O
-
 Clostridium botulinum TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQ + KLSELDDRADAL
-
 ?
TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADAL + H2O
-
 Clostridium baratii TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQ + KLSELDDRADAL
-
 ?
TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADAL + H2O
-
 Clostridium baratii CNM1212/11 TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQ + KLSELDDRADAL
-
 ?
TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADAL + H2O
-
 Clostridium botulinum NCTC 10281 TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQ + KLSELDDRADAL
-
 ?
TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADAL + H2O
-
 Clostridium botulinum H078-01 TSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQ + KLSELDDRADAL
-
 ?
vesicle-associated membrane protein-2 + H2O cleavage at Gln58-Lys59 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-2 + H2O cleavage at Gln58-Lys59 Clostridium baratii ?
-
 ?
vesicle-associated membrane protein-2 + H2O cleavage at Gln58-Lys59 Clostridium baratii CNM1212/11 ?
-
 ?
vesicle-associated membrane protein-2 + H2O cleavage at Gln58-Lys59 Clostridium botulinum NCTC 10281 ?
-
 ?
vesicle-associated membrane protein-2 + H2O cleavage at Gln58-Lys59 Clostridium botulinum H078-01 ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/F1 subtype Clostridium botulinum
BoNT/F6 subtype Clostridium botulinum
BoNT/F7 subtype Clostridium baratii
BoNT/F9 subtype Clostridium botulinum
Botulinum neurotoxin
-
 Clostridium botulinum
Botulinum neurotoxin
-
 Clostridium baratii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.37
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F6, at pH 6.8 and 37°C Clostridium botulinum
12.43
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F9, at pH 6.8 and 37°C Clostridium botulinum
23.25
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F1, at pH 6.8 and 37°C Clostridium botulinum
35
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F7, at pH 6.8 and 37°C Clostridium baratii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
281.7
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F9, at pH 6.8 and 37°C Clostridium botulinum
465
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F7, at pH 6.8 and 37°C Clostridium baratii
810
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F1, at pH 6.8 and 37°C Clostridium botulinum
1050
-
 vesicle-associated membrane protein-2 enzyme subtype BoNT/F6, at pH 6.8 and 37°C Clostridium botulinum