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Literature summary for 3.4.24.69 extracted from

  • Masuyer, G.; Zhang, S.; Barkho, S.; Shen, Y.; Henriksson, L.; Ko?enina, S.; Dong, M.; Stenmark, P.
    Structural characterisation of the catalytic domain of botulinum neurotoxin X - high activity and unique substrate specificity (2018), Sci. Rep., 8, 4518 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using either 19% (w/v) PEG1500, 0.1 M MMT pH 6.0 or 11% (w/v) PEG20000, 0.1 M MES pH 6.5, 10 mM EDTA Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
 vesicle-associated membrane protein-1 at pH 7.1 and 37°C Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vesicle-associated membrane protein-1 + H2O Clostridium botulinum the enzyme suybtype BoNT/X cleaves vesicle-associated membrane protein-1 with a 10times higher efficiency than subtype BoNT/B and tetanus neurotoxin ?
-
 ?
vesicle-associated membrane protein-2 + H2O Clostridium botulinum
-
 ?
-
 ?
vesicle-associated membrane protein-3 + H2O Clostridium botulinum
-
 ?
-
 ?
vesicle-associated membrane protein-4 + H2O Clostridium botulinum
-
 ?
-
 ?
vesicle-associated membrane protein-5 + H2O Clostridium botulinum
-
 ?
-
 ?
Ykt6 + H2O Clostridium botulinum
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum P0DPK1
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S200 gel filtration Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
vesicle-associated membrane protein-1 + H2O the enzyme suybtype BoNT/X cleaves vesicle-associated membrane protein-1 with a 10times higher efficiency than subtype BoNT/B and tetanus neurotoxin Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-2 + H2O
-
 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-3 + H2O
-
 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-4 + H2O
-
 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-5 + H2O
-
 Clostridium botulinum ?
-
 ?
Ykt6 + H2O
-
 Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/X
-
 Clostridium botulinum
botulinum neurotoxin X
-
 Clostridium botulinum
LC/X catalytic domain/light chain of botulinum neurotoxin X Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.52
-
 vesicle-associated membrane protein-1 at pH 7.1 and 37°C Clostridium botulinum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1050
-
 vesicle-associated membrane protein-1 at pH 7.1 and 37°C Clostridium botulinum