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Literature summary for 3.4.24.69 extracted from

  • Masuyer, G.; Davies, J.R.; Moore, K.; Chaddock, J.A.; Ravi Acharya, K.
    Structural analysis of Clostridium botulinum neurotoxin type D as a platform for the development of targeted secretion inhibitors (2015), Sci. Rep., 5, 13397 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment) Organism
catalytic and translocation domain of botulinum neurotoxin type D, sitting drop vapor diffusion method, using 0.1 M sodium actetate pH 5.5, 0.8 M sodium formate, 10% (w/v) polyethylene glycol 8000, 10% (w/v) polyethylene glycol 1000 Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
H233Y/E230Q inactive Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
synaptosome-associated protein SNAP-25 + H2O Clostridium botulinum
-
 ?
-
 ?
Syntaxin + H2O Clostridium botulinum
-
 ?
-
 ?
vesicle-associated membrane protein-2 + H2O Clostridium botulinum
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum P19321
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-charged chelating Sepharose column chromatography and phenyl Sepharose column chromatography Clostridium botulinum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
synaptosome-associated protein SNAP-25 + H2O
-
 Clostridium botulinum ?
-
 ?
Syntaxin + H2O
-
 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-2 + H2O
-
 Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/D
-
 Clostridium botulinum
botulinum neurotoxin type D
-
 Clostridium botulinum
LHn/D catalytic and translocation domain of botulinum neurotoxin type D Clostridium botulinum