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Literature summary for 3.4.24.69 extracted from

  • Lam, K.H.; Sikorra, S.; Weisemann, J.; Maatsch, H.; Perry, K.; Rummel, A.; Binz, T.; Jin, R.
    Structural and biochemical characterization of the protease domain of the mosaic botulinum neurotoxin type HA (2018), Pathog. Dis., 76, fty044 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Star (DE3) cells Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment) Organism
light chain protease domain of botulinum neurotoxin type HA, hanging drop vapor diffusion method, using 100 mM calcium acetate, 100 mM sodium cacodylate, pH 5.5 and 4% (w/v) polyethylene glycol 8000 Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
Q34A the mutation reduces the VAMP-2 cleavability by about 3fold compared to the wild type enzyme Clostridium botulinum
S28E the mutation reduces the VAMP-2 cleavability by about 2fold compared to the wild type enzyme Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2535
-
 vesicle-associated membrane protein-2 pH and temperature not specified in the publication Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vesicle-associated membrane protein-2 + H2O Clostridium botulinum cleavage between L54 and E55 ?
-
 ?
vesicle-associated membrane protein-2 + H2O Clostridium botulinum BCA10-7060 cleavage between L54 and E55 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-
Clostridium botulinum BCA10-7060
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST-affinity column chromatography and Superdex S200 gel filtration Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
vesicle-associated membrane protein-2 + H2O cleavage between L54 and E55 Clostridium botulinum ?
-
 ?
vesicle-associated membrane protein-2 + H2O cleavage between L54 and E55 Clostridium botulinum BCA10-7060 ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/HA
-
 Clostridium botulinum
botulinum neurotoxin type HA
-
 Clostridium botulinum
LC/HA light chain protease domain of botulinum neurotoxin type HA Clostridium botulinum
mosaic toxin
-
 Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.3
-
 vesicle-associated membrane protein-2 pH and temperature not specified in the publication Clostridium botulinum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
68.3
-
 vesicle-associated membrane protein-2 pH and temperature not specified in the publication Clostridium botulinum