Literature summary for 3.4.24.69 extracted from

Davies, J.R.; Rees, J.; Liu, S.M.; Acharya, K.R.
High resolution crystal structures of Clostridium botulinum neurotoxin A3 and A4 binding domains (2018), J. Struct. Biol., 202, 113-117 .

Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment)	Organism
binding domain of subtype BoNT/, sitting drop vapor diffusion method, using 10 mM spermine tetrahydrochloride, 10 mM spermidine trihydrochloride, 10 mM 1,4-diaminobutane dihydrochloride, 10 mM DL-ornithine monohydrochloride, 0.1 M MOPSO/bis-Tris pH 6.5, 15% (w/v) PEG 3 K, 20% (w/v), 1,2,4-butanetriol, 1% (w/v) NDSB 256	Clostridium botulinum
binding domain of subtype BoNT/A4, sitting drop vapor diffusion method, using 10 mM spermine tetrahydrochloride, 10 mM spermidine trihydrochloride, 10 mM 1,4-diaminobutane dihydrochloride, 10 mM DL-ornithine monohydrochloride, 0.1 M MOPSO/bis-Tris pH 6.5, 15% (w/v) PEG 3 K, 20% (w/v), 1,2,4-butanetriol, 1% (w/v) NDSB 256	Clostridium botulinum

Organism

Organism	UniProt	Comment	Textmining
Clostridium botulinum	Q3LRX8	-	-
Clostridium botulinum	Q3LRX9	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex S200 gel filtration	Clostridium botulinum

Synonyms

Synonyms	Comment	Organism
BoNT/A3	-	Clostridium botulinum
BoNT/A4	-	Clostridium botulinum
botulinum neurotoxin A3	-	Clostridium botulinum
botulinum neurotoxin A4	-	Clostridium botulinum

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Release 2023.1 (January 2023)