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Literature summary for 3.4.24.69 extracted from

  • Sikorra, S.; Litschko, C.; Mueller, C.; Thiel, N.; Galli, T.; Eichner, T.; Binz, T.
    Identification and characterization of botulinum neurotoxin A substrate binding pockets and their re-engineering for human SNAP-23 (2016), J. Mol. Biol., 428, 372-384 .
    View publication on PubMed
Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15pREP4 cells Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
A308L the mutant shows 8.7% of wild type SNAP-25 cleavage activity Clostridium botulinum
A308V the mutant shows 86.4% of wild type SNAP-25 cleavage activity Clostridium botulinum
E148A the mutant shows 8.2% of wild type SNAP-25 cleavage activity Clostridium botulinum
E148K the mutant shows 1.7% of wild type SNAP-25 cleavage activity Clostridium botulinum
E148R the mutant shows 4.0% of wild type SNAP-25 cleavage activity Clostridium botulinum
K23A the mutant shows 111% of wild type SNAP-25 cleavage activity Clostridium botulinum
K23D the mutant shows 72% of wild type SNAP-25 cleavage activity Clostridium botulinum
K337A the mutant shows 55% of wild type SNAP-25 cleavage activity Clostridium botulinum
K337E the mutant shows 28.5% of wild type SNAP-25 cleavage activity Clostridium botulinum
K340A the mutant shows 99.5% of wild type SNAP-25 cleavage activity Clostridium botulinum
K340D the mutant shows 71.5% of wild type SNAP-25 cleavage activity Clostridium botulinum
K340H the mutant shows 64.1% of wild type SNAP-25 cleavage activity Clostridium botulinum
K340R the mutant shows 147.5% of wild type SNAP-25 cleavage activity Clostridium botulinum
L256E the mutant shows 13.9% of wild type SNAP-25 cleavage activity Clostridium botulinum
L256E/V258P the mutant shows 49.3% of wild type SNAP-25 cleavage activity Clostridium botulinum
M106A the mutant shows 73% of wild type SNAP-25 cleavage activity Clostridium botulinum
M344A the mutant shows 73% of wild type SNAP-25 cleavage activity Clostridium botulinum
N26D the mutant shows 90.8% of wild type SNAP-25 cleavage activity Clostridium botulinum
N26K the mutant shows 82.8% of wild type SNAP-25 cleavage activity Clostridium botulinum
Q162A the mutant shows 110% of wild type SNAP-25 cleavage activity Clostridium botulinum
R177A the mutant shows 82% of wild type SNAP-25 cleavage activity Clostridium botulinum
R177D the mutant shows 77% of wild type SNAP-25 cleavage activity Clostridium botulinum
T132D the mutant shows 42.5% of wild type SNAP-25 cleavage activity Clostridium botulinum
V258A the mutant shows 33.1% of wild type SNAP-25 cleavage activity Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SNAP-25 + H2O Clostridium botulinum
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose bead chromatography Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with human SNAP-23 Clostridium botulinum ?
-
 ?
SNAP-25 + H2O
-
 Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/A
-
 Clostridium botulinum
botulinum neurotoxin a
-
 Clostridium botulinum
LCA
-
 Clostridium botulinum