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Literature summary for 3.4.24.69 extracted from
- Expression and biochemical characterization of light chains of Botulinum neurotoxin subtypes F5 and F7 (2015), Protein Expr. Purif., 111, 87-90.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Clostridium botulinum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0172 | - |
VAMP-2 | subtype BoNT/F5, untagged, pH and temperature not specified in the publication | Clostridium botulinum | |
| 0.0231 | - |
VAMP-2 | His6-tagged subtype BoNT/F7, pH and temperature not specified in the publication | Clostridium botulinum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc-dependent protease | Clostridium botulinum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | Clostridium botulinum | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
| Clostridium botulinum | D2KHS9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | - |
Clostridium botulinum | ? | - |
? | |
| VAMP-2 + H2O | - |
Clostridium botulinum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * about 100000 + 1 * about 60000, subtype BoNT/F7, SDS-PAGE | Clostridium botulinum |
| heterodimer | 1 * about 100000 + 1 * about 70000, subtype BoNT/F5, SDS-PAGE | Clostridium botulinum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/F5 | subtype | Clostridium botulinum |
| BoNT/F7 | subtype | Clostridium botulinum |
| Botulinum neurotoxin | - |
Clostridium botulinum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 64.69 | - |
VAMP-2 | His6-tagged subtype BoNT/F7, pH and temperature not specified in the publication | Clostridium botulinum | |
| 594.6 | - |
VAMP-2 | subtype BoNT/F5, untagged, pH and temperature not specified in the publication | Clostridium botulinum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2800 | - |
VAMP-2 | His6-tagged subtype BoNT/F7, pH and temperature not specified in the publication | Clostridium botulinum | |
| 34570 | - |
VAMP-2 | subtype BoNT/F5, untagged, pH and temperature not specified in the publication | Clostridium botulinum |
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