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Literature summary for 3.4.24.69 extracted from
- Light chain separated from the rest of the type a botulinum neurotoxin molecule is the most catalytically active form (2010), PLoS ONE, 5, e12872.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | four variants of type A BoNT (BoNT/A) light chain are prepared and their catalytic parameters with those of BoNT/A whole toxin are compared. The four variants are light chain + translocation domain, a trypsin-nicked light chain + translocation domain, light chain + belt, and a free light chain. Km (SNAP-25) (synaptosomal associated protein of 25 kDa) for these constructs is not very different, but kcat for the free light chain is 6-100fold higher than those of its four variants. None of the four variants of the light chain is prone to autocatalysis | Clostridium botulinum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
SNAP-25 | pH 7.4, 23°C, mutant light chain + translocation domain | Clostridium botulinum | |
| 1.3 | - |
SNAP-25 | pH 7.4, 23°C, mutant light chain + belt | Clostridium botulinum | |
| 3.36 | - |
SNAP-25 | pH 7.4, 23°C, mutant light-chain | Clostridium botulinum | |
| 4.29 | - |
SNAP-25 | pH 7.4, 23°C, wild-type | Clostridium botulinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | 17-residue C-terminal peptide corresponding to residue 187-203 of SNAP-25 | Clostridium botulinum | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/A | - |
Clostridium botulinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at for determination of Km and kcat | Clostridium botulinum |
| 37 | - |
assay at | Clostridium botulinum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
SNAP-25 | pH 7.4, 23°C, mutant light chain + translocation domain | Clostridium botulinum | |
| 0.31 | - |
SNAP-25 | pH 7.4, 23°C, mutant light chain + belt | Clostridium botulinum | |
| 1.42 | - |
SNAP-25 | pH 7.4, 23°C, wild-type | Clostridium botulinum | |
| 9 | - |
SNAP-25 | pH 7.4, 23°C, mutant light-chain | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Clostridium botulinum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
SNAP-25 | pH 7.4, 23°C, mutant light chain + translocation domain | Clostridium botulinum | |
| 0.24 | - |
SNAP-25 | pH 7.4, 23°C, mutant light chain + belt | Clostridium botulinum | |
| 0.33 | - |
SNAP-25 | pH 7.4, 23°C, wild-type | Clostridium botulinum | |
| 2.68 | - |
SNAP-25 | pH 7.4, 23°C, mutant light-chain | Clostridium botulinum |
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