Literature summary for 3.4.24.69 extracted from

Benson, M.A.; Fu, Z.; Kim, J.J.; Baldwin, M.R.
Unique ganglioside recognition strategies for clostridial neurotoxins (2011), J. Biol. Chem., 286, 34015-34022.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structure of the BoNT/F receptor-binding domain in complex with the sugar moiety of ganglioside GD1a is reported. GD1a binds in a shallow groove formed by a conserved peptide motif, with additional stabilizing interactions provided by two arginine residues	Clostridium botulinum

Protein Variants

Protein Variants	Comment	Organism
H1241K	mutant shows an increased affinity for GD1a and confers the ability to bind ganglioside GM1a	Clostridium botulinum
R1111A/H1241K/R1256A	triple mutant binds GD1a 17fold greater than the double-mutant	Clostridium botulinum
R1111A/R1256A	triple mutant binds GD1a 17fold greater than the double-mutant	Clostridium botulinum

Organism

Organism	UniProt	Comment	Textmining
Clostridium botulinum	Q57236	-	-

Synonyms

Synonyms	Comment	Organism
BoNT/F	-	Clostridium botulinum

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Release 2023.1 (January 2023)