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Literature summary for 3.4.24.69 extracted from

  • Pires-Alves, M.; Ho, M.; Aberle, K.K.; Janda, K.D.; Wilson, B.A.
    Tandem fluorescent proteins as enhanced FRET-based substrates for botulinum neurotoxin activity (2009), Toxicon, 53, 392-399.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium botulinum the catalytic light chains of BoNTs, BoNT-LC, recognize extended regions of their substrates for cleavage ?
-
 ?
SNAP-25 + H2O Clostridium botulinum i.e. 25 kDa synaptosome-associated protein, BoNT/A requires two extended exosites for optimal substrate binding and recognition of its intracellular target SNAP-25 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the catalytic light chains of BoNTs, BoNT-LC, recognize extended regions of their substrates for cleavage Clostridium botulinum ?
-
 ?
additional information feasibility of using the CFP-YFP pair with full-length SNAP-25 as a FRET-based substrate for BoNT/A in a cell-based assay or with the 66-mer peptide as a FRET substrate in an in vitro assay, optimization of FRET efficiency by use of fluorescent protein variants, CsY, CsYY or YsCsY, overview Clostridium botulinum ?
-
 ?
SNAP-25 + H2O i.e. 25 kDa synaptosome-associated protein, BoNT/A requires two extended exosites for optimal substrate binding and recognition of its intracellular target SNAP-25 Clostridium botulinum ?
-
 ?
SNAP-25 peptide (141-206) + H2O the minimal size of SNAP-25 known to retain full activity as a BoNT/A substrate is the C-terminal 66-mer peptide, residues 141-206, with both exosites Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/A
-
 Clostridium botulinum
Botulinum neurotoxin
-
 Clostridium botulinum
botulinum neurotoxin a
-
 Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium botulinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Clostridium botulinum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information the non-fluorescent substrate GST-SNAP-25(141-206) is a competitive inhibitor in the fluorometric assay for CsY and YsCsY with Ki values of 0.0076 and 0.0045 mM, respectively Clostridium botulinum