Literature summary for 3.4.24.69 extracted from

Rummel, A.; Haefner, K.; Mahrhold, S.; Darashchonak, N.; Holt, M.; Jahn, R.; Beermann, S.; Karnath, T.; Bigalke, H.; Binz, T.
Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor (2009), J. Neurochem., 110, 1942-1954.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strains BL21(DE3) and M15 of GST-tagged HC-fragments pHCAS, pHCBS, pHCCS, pHCDS, pHCES, pHCFS, and pHCGS, and the full-length BoNT/A, BoNT/B, BoNT/D, BoNT/G, as well as pBoNTCS-Thro encoding full-length BoNT/C fused C-terminally to a Streptag and containing an Escherichia coli protease sensitive peptide between LC and HC	Clostridium botulinum

Protein Variants

Protein Variants	Comment	Organism
E1172A	the binding of radioactively-labeled, in vitro translated HCE E1172A to rat brain synaptosomes is highly decreased to 8.5% of wild-type levels, as well as the binding of Escherichia coli derived HCE mutants to isolated GT1b	Clostridium botulinum
E1195A	the HCF mutant E1195A displays a diminished affinity of 31.6% to synaptosomes as well as a reduction of about 85% in binding to isolated GT1b compared to HCF wild-type	Clostridium botulinum
W1224L	the binding of radioactively-labeled, in vitro translated HCE W1224L to rat brain synaptosomes is highly decreased to 3.7% of wild-type levels, as well as the binding of Escherichia coli derived HCE mutants to isolated GT1b	Clostridium botulinum
W1250L	the HCF mutant W1250L displays a diminished affinity of 20.5% to synaptosomes as well as a reduction of about 85% in binding to isolated GT1b compared to HCF wild-type	Clostridium botulinum
W1266L	the mutant HCA W1266L that lacks ganglioside binding, does not interfere with BoNT/A neurotoxicity	Clostridium botulinum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the toxicity of the enzyme is reduced in absence of gangliosides	Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Clostridium botulinum	BoNT serotypes bind to structure of ganglioside GT1b receptors, structure and binding specificities, modelling, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Clostridium botulinum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged HC-fragments pHCAS, pHCBS, pHCCS, pHCDS, pHCES, pHCFS, and pHCGS, and the full-length BoNT/A, BoNT/B, BoNT/D, BoNT/G, and Strep-tagged pBoNTCS-Thro encoding from Escherichia coli by glutathione and streptavidin affinity chromatography, respectively	Clostridium botulinum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	BoNT serotypes bind to structure of ganglioside GT1b receptors, structure and binding specificities, modelling, overview	Clostridium botulinum	?	-	?

Subunits

Subunits	Comment	Organism
More	the BoNT toxin is composed of a catalytic light chain, a heavy chain, and a translocation domain	Clostridium botulinum

Synonyms

Synonyms	Comment	Organism
BoNT/A	-	Clostridium botulinum
BoNT/B	-	Clostridium botulinum
BoNT/C	-	Clostridium botulinum
BoNT/D	-	Clostridium botulinum
BoNT/E	-	Clostridium botulinum
BoNT/F	-	Clostridium botulinum
BoNT/G	-	Clostridium botulinum
botulinum neurotoxin C	-	Clostridium botulinum
botulinum neurotoxin E	-	Clostridium botulinum
botulinum neurotoxin F	-	Clostridium botulinum

General Information

General Information	Comment	Organism
additional information	uptake of BoNT/A, B, E and G into cells requires a dual interaction with gangliosides and the synaptic vesicle proteins synaptotagmin or SV2. Interaction of SV2A, SV2B and SV2C with botulinum neurotoxin F HC, overview	Clostridium botulinum

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Release 2023.1 (January 2023)