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Literature summary for 3.4.24.69 extracted from

  • Nuss, J.E.; Ruthel, G.; Tressler, L.E.; Wanner, L.M.; Torres-Melendez, E.; Hale, M.L.; Bavari, S.
    Development of cell-based assays to measure botulinum neurotoxin serotype A activity using cleavage-sensitive antibodies (2010), J. Biomol. Screen., 15, 42-51.
    View publication on PubMed
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Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc-metalloprotease Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SNAP-25 + H2O i.e. 25-kDa synaptosome-associated protein, development of a BoNT/A-specific assay method, overview. Usage of BoNT/A cleavage-sensitive antibodies that only interact with full-length SNAP-25, the molecular target of the BoNT/A serotype. These antibodies exhibit high specificity for full-length SNAP-25, allowing the BoNT/A-mediated proteolysis of this protein to be measured in diverse assay formats, e.g. ELISA and immunofluorescent assay methods, detailed overview Clostridium botulinum ?
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 ?

Synonyms

Synonyms Comment Organism
BoNT/A
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 Clostridium botulinum
botulinum neurotoxin serotype A
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 Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium botulinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Clostridium botulinum

General Information

General Information Comment Organism
physiological function botulinum neurotoxins cleave components of the SNARE, soluble N-ethylmaleimide-sensitive factor attachment protein receptor, protein complex, inhibiting acetylcholine release into neuromuscular junctions, resulting in flaccid paralysis and eventual death Clostridium botulinum