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Literature summary for 3.4.24.69 extracted from

  • Thanongsaksrikul, J.; Srimanote, P.; Maneewatch, S.; Choowongkomon, K.; Tapchaisri, P.; Makino, S.; Kurazono, H.; Chaicumpa, W.
    A V H H that neutralizes the zinc metalloproteinase activity of botulinum neurotoxin type A (2010), J. Biol. Chem., 285, 9657-9666.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Application

Application Comment Organism
drug development the botulinum toxin is a target for development of specific drugs to treat botulism Clostridium botulinum

Inhibitors

Inhibitors Comment Organism Structure
additional information construction of type A-specific monoclonal antibodies using antigene heavy chain antibody fragment VH/VHH from a nonimmune camel, primers specific to human VH gene segments, and recombinant expression in phagemid-transformed Escherichia coli. The selected antibodies inhibiting the endopeptidase activity of BoTxA light chain and of botulism in hosts in vivo. Molecular docking and interface binding of BoTxA/LC and antibody VHH17 Clostridium botulinum
PTEN a zinc-chelating agent Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-dependent metalloprotease Clostridium botulinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, type A botulinum neurotoxin light chain, SDS-PAGE Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SNAP-25 + H2O Clostridium botulinum i.e. 25-kDa synaptosome-associated protein ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum P10845
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SNAP-25 + H2O i.e. 25-kDa synaptosome-associated protein Clostridium botulinum ?
-
 ?
SNRTRIDEANK(Dnp)RA(S-(N-[4-methyl-7-dimethylamino-coumarin-3-yl]-carboxamidomethyl)-L-cysteine)RML + H2O a synthetic fluorogenic peptide substrate of BoTxA/LC, representing amino acid residues 187-203 of SNAP25, a cleavage site of the enzyme Clostridium botulinum SNRTRIDEANK(Dnp) + RA(S-(N-[4-methyl-7-dimethylamino-coumarin-3-yl]-carboxamidomethyl)-L-cysteine)RML
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, type A botulinum neurotoxin light chain, SDS-PAGE Clostridium botulinum

Synonyms

Synonyms Comment Organism
botulinum neurotoxin type A
-
 Clostridium botulinum
BoTxA
-
 Clostridium botulinum
type A botulinum neurotoxin
-
 Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium botulinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Clostridium botulinum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information binding and inhibition kinetics of antigene heavy chain antibody fragment VH/VHH-specific antibodies, overview Clostridium botulinum