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Literature summary for 3.4.24.69 extracted from
- An assay for botulinum toxin types A, B and F that requires both functional binding and catalytic activities within the neurotoxin (2009), J. Appl. Microbiol., 107, 1384-1391.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | Clostridium botulinum | i.e. 25-kDa synaptosome-associated protein, BoNT/A | ? | - |
? |  |
| VAMP + H2O | Clostridium botulinum | i.e. vesicle associated membrane protein or synaptobrevin, BoNT/B, and BoNT/F | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
synaptosome capture assay for the different serotype BoNTs, synaptosome from rat brains, overview | Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | synaptosome capture assay for the different serotype BoNTs, synaptosome from rat brains, overview | Clostridium botulinum | ? | - |
? | |
| SNAP-25 + H2O | i.e. 25-kDa synaptosome-associated protein, BoNT/A | Clostridium botulinum | ? | - |
? | |
| VAMP + H2O | i.e. vesicle associated membrane protein or synaptobrevin, BoNT/B, and BoNT/F | Clostridium botulinum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/A1 | - |
Clostridium botulinum |
| BoNT/A2 | - |
Clostridium botulinum |
| BoNT/A3 | - |
Clostridium botulinum |
| BoNT/B1 | - |
Clostridium botulinum |
| BoNT/F | - |
Clostridium botulinum |
| botulinum toxin type A | - |
Clostridium botulinum |
| botulinum toxin type B | - |
Clostridium botulinum |
| botulinum toxin type F | - |
Clostridium botulinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
different serotypes were found to possess different optimal cleavage pHs | Clostridium botulinum |
| 6.5 | - |
BoNT/A1 | Clostridium botulinum |
| 7.4 | - |
BoNT/B1 | Clostridium botulinum |
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Release 2023.1 (January 2023)
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