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Literature summary for 3.4.24.69 extracted from
- Antibody protection against botulinum neurotoxin intoxication in mice (2009), Infect. Immun., 77, 4305-4313.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | ability of monoclonal antibodies F1-2 and F1-40 to provide therapeutic protection against BoNT/A intoxication in mouse intravenous and oral intoxication models, overview | Clostridium botulinum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E224A | the mutation abrogates the catalytic activity of the endopeptidase of BoNT/A | Clostridium botulinum |
| H223A | the mutation abrogates the catalytic activity of the endopeptidase of BoNT/A | Clostridium botulinum |
| H227A | the mutation abrogates the catalytic activity of the endopeptidase of BoNT/A | Clostridium botulinum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | monoclonal antibodies F1-2 and F1-40 do not inhibit the catalytic activity of BoNT/A, but inhibit the entry of the toxin into host neuronal cells, leading to reduced toxicity to mice, and block intracellular SNAP25 cleavage, overview | Clostridium botulinum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Clostridium botulinum |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | i.e. 25-kDa synaptosome-associated protein | Clostridium botulinum | ? | - |
? | |
| SNAPtide + H2O | i.e. SNAPtide, as recombinant human SNAP25bHA protein expressed in Escherichia coli | Clostridium botulinum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT serotype A | - |
Clostridium botulinum |
| BoNT/A | - |
Clostridium botulinum |
| Botulinum neurotoxin | - |
Clostridium botulinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Clostridium botulinum |
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Release 2023.1 (January 2023)
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