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Literature summary for 3.4.24.69 extracted from
- Rapid multiplexed flow cytometric assay for botulinum neurotoxin detection using an automated fluidic microbead-trapping flow cell for enhanced sensitivity (2009), Anal. Chem., 81, 5783-5793.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
serotype A BoNT | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | functional assay of the toxin protease activity using a fluorogenic substrate. Development of a bead-based sandwich immunoassay for botulinum neurotoxin serotype A, BoNT/A, using a recombinant 50 kDa fragment of the BoNT/A heavy chain as a structurally valid simulant. Different anti-BoNT/A antibodies are attached to three different fluorescent, dye encoded flow cytometry beads for multiplexing. The assay is conducted in two formats: a manual microcentrifuge tube format and an automated fluidic system format. Flow cytometry detection is used for both formats, method evaluation, overview | Clostridium botulinum | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/A | - |
Clostridium botulinum |
| Botulinum neurotoxin | - |
Clostridium botulinum |
| botulinum neurotoxin serotype A | - |
Clostridium botulinum |
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