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Literature summary for 3.4.24.69 extracted from
- High level expression of the light chain of botulinum neurotoxin serotype C1 and an efficient HPLC assay to monitor its proteolytic activity (2008), Protein Expr. Purif., 60, 165-169.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | essential for activity of BoNT/C1 | Clostridium botulinum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| high level overexpression by autoinduction method of botulinum neurotoxin serotype C1, comprising residues 1-430, in Escherichia coli strain BL21(DE3) as His-tagged protein | Clostridium botulinum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc metalloprotease | Clostridium botulinum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP25 + H2O | Clostridium botulinum | i.e. synaptosomal-associated protein of 25 kDa | ? | - |
? | |
| Syntaxin + H2O | Clostridium botulinum | - |
? | - |
? | |
| VAMP + H2O | Clostridium botulinum | i.e. vesicle associated membrane protein | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged BoNT/C1(1-430) from Escherichia coli strain B21(DE3) by nickel affinity chromatography | Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the neurotoxin serotypes show distinct substrate specificities, overview | Clostridium botulinum | ? | - |
? | |
| SNAP25 + H2O | i.e. synaptosomal-associated protein of 25 kDa | Clostridium botulinum | ? | - |
? | |
| SNAP25 + H2O | i.e. synaptosomal-associated protein of 25 kDa, SNAP25 with varying peptide length, substrate specificity of BoNT/C1, e.g. 17mer peptide corresponding to residues 187-203 of SNAP-25 is a substrate for BoNT/C1 (1-430), importance of remote exosites in BoNT/C1 required for activity, assay optimization, overview | Clostridium botulinum | ? | - |
? | |
| Syntaxin + H2O | - |
Clostridium botulinum | ? | - |
? | |
| VAMP + H2O | i.e. vesicle associated membrane protein | Clostridium botulinum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT | - |
Clostridium botulinum |
| BoNT/C1 | - |
Clostridium botulinum |
| Botulinum neurotoxin | - |
Clostridium botulinum |
| botulinum neurotoxin serotype A | - |
Clostridium botulinum |
| botulinum neurotoxin serotype B | - |
Clostridium botulinum |
| botulinum neurotoxin serotype C1 | - |
Clostridium botulinum |
| botulinum neurotoxin serotype D | - |
Clostridium botulinum |
| botulinum neurotoxin serotype E | - |
Clostridium botulinum |
| botulinum neurotoxin serotype F | - |
Clostridium botulinum |
| botulinum neurotoxin serotype G | - |
Clostridium botulinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Clostridium botulinum |
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Release 2023.1 (January 2023)
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